Nephelometric Method for Determination of Growth Parameters of Chlorella Culture
Abstract
Nephelometric method for the determination of growth parameters of chlorella culture using a photoelectric colorimeter was described. Use of photoelectric colorimeter for cell counting in suspension requires periodic calibration of meter readings using chlorella standard culture (with a certain cell concentration). Chlorella vulgaris IPPAS С-66, IPPAS С-111 and IPPAS С-2019 strains served as object of research. Density of initial in vitro suspensions (after inoculation) was 0.9 mln cells/ml. Cultivation was carried out during 12 days on a Hoagland medium with a pH of 7 (temperature of 35∘C, illumination of 10 klx). Sample selection for analysis and measurement was carried out daily, three times per day. Based on the obtained data, readings of photoelectric colorimeter KFK-3.01 were calibrated via direct count of chlorella cells quantity in Goryaev’s chamber. Use of calibration curve made it possible to reduce significantly time and error in determination of cell number in suspension cultures. The proposed technique allows counting chlorella cells in the growth and development dynamics with sufficient accuracy, high sensitivity, reproducibility and speed. It can be used for comparative determination of the growth parameters of strains in vitro, standardization of suspension cultures, semi-quantitative determination of chlorella biomass in order to predict the yield of desired product.
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