Analysis of Phorbolester Content During Growth and Development of <i>Jatropha curcas</i> Fruits
Abstract
Utilization of Jatropha curcas seed meal as animal feed is limited by the presence of toxic compounds. Phorbolester present in Jatropha curcas as a family of compounds known to cause a large number of biological effects such as tumor promoters. The aim of this research is measure phorbolester content during growth and development of Jatropha curcas fruit. Phorbolester extracted by sonification method and analysed by UPLC using phosphoric acid and acetonitrile as mobile phase. Jatropha curcas fruit has ripe in the fifth week because of yellowing of fruit capsule. Toxic genotypes of Jatropha curcas has higher phorobol ester content than non-toxic genotypes approximately 182.1 and 55.2 ng/g respectively. Fruit capsule (122.2 ng/g) has higher phorbolester content than fruit seed (115.2 ng/g). It is due to biosynthesis of diterpene that occur in plastid. Biosynthesis of phorbolester possibly occured in initiation and maturation of fruit because the high level of phorbolester occur in the first and fifth week.
Keywords: Jatropha curcas, toxic genotypes, non-toxic genotypes, fruit seed, fruit capsule
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