Efficient Encoding of Matrix Microparticles with Nanocrystals for Fluorescent Polyelectrolyte Microcapsules Development

Abstract

Polyelectrolyte microcapsules development and further use as specific carriers for drug molecules, fluorescent dyes, and metal nanoparticles is a promising approach to designing theranostic agents. Semiconductor nanocrystal quantum dots exhibiting size-dependent optical properties, a high photostability, and optimal fluorescent properties can be advantageous over classical organic fluorophores. The results of elaboration of efficient encoding of matrix microparticles with nanocrystals for development of fluorescent polyelectrolyte microcapsules and the characteristics of the obtained encoded microbeads are demonstrated.


Keywords: Semiconductor nanocrystals; encoding of matrix microbeads; theranostic agents, polyelectrolyte microcapsules, layer-by-layer technique.

References
[1] Xiong R., Soenen S.J., Braeckmans K., Skirtach A.G. (2013) Towards theranostic multicompartment microcapsules: in-situ diagnostics and laser-induced treatment, Theranostics, 3, 141-151. DOI: 10.7150/thno.5846.


[2] Yu W., Chen Y., and Mao Zh. (2016) Hollow Polyelectrolyte Microcapsules as Advanced Drug Delivery Carriers, Journal of Nanoscience and Nanotechnology, 16, 5435–5446. DOI: 10.1166/jnn.2016.11748.


[3] De Geest B.G., De Koker S., Sukhorukov G.B., Kreft O., Parak W.J., Skirtach A.G. , Demeester J., De Smedt S.C., Hennink W.E. (2009) Polyelectrolyte microcapsules for biomedical applications, Soft Matter, 5, 282–291. DOI: 10.1039/B808262F.


[4] Zhao Q., Li B. (2008) pH-controlled drug loading and release from biodegradable microcapsules, Nanomedicine: NBM, 4, 302–310. DOI: 10.1016/j.nano.2008.06.004.


[5] Bilan R., Ametzazurra A., Brazhnik K., Escorza S., Fernández D., Uríbarri M., Nabiev I., Sukhanova A. (2017) Quantum-dot-based suspension microarray for multiplex detection of lung cancer markers: preclinical validation and comparison with the Luminex xMAP® system, Sci. Rep., 7, 44668. DOI: 10.1038/srep44668.


[6] Brazhnik K., Sokolova Z., Baryshnikova М., Bilan R., Efimov A., Nabiev I., Sukhanova A. (2015) Quantum dot-based lab-on-a-bead system for multiplexed detection of free and total prostate-specific antigens in clinical human serum samples, Nanomedicine: NBM, 11, 1065-1075. DOI: 10.1016/j.nano.2015.03.003.


[7] Sukhorukov G.B., Volodkin D.V., Günther A.M., Petrov A.I., Shenoy D.B., Möhwald H. (2004) Porous calcium carbonate microparticles as templates for encapsulation of bioactive compounds, J. Mater. Chem., 14, 2073–2081. DOI: 10.1039/B402617A.


[8] Bilan R.S., Krivenkov V.A., Berestovoy M.A., Efimov A.E., Agapov I.I., Samokhvalov P.S., Nabiev I., Sukhanova A. (2017) Engineering of optically encoded microbeads with FRET-free spatially separated quantum dot layers for multiplexed assays, ChemPhysChem, 18, 970-979. DOI: 10.1002/cphc.201601274.