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Mehdi Soltan Dallal M, Nikkhahi F, Mostafa Imeni S, Molaei S, Kazem Hosseini S, Kalafi Z, Sharifi Yazdi S, Molla Agha Mirzaei H. Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model. JOVR [Internet]. 2021Oct.25 [cited 2024Apr.20];16(4):552–557. Available from: https://knepublishing.com/index.php/JOVR/article/view/9744
https://doi.org/10.18502/jovr.v16i4.9744
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authors
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Mohammad Mehdi Soltan Dallal
Mohammad Mehdi Soltan Dallal
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran
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Farhad Nikkhahi
Farhad Nikkhahi
Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin, Iran
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Seyed Mostafa Imeni
Seyed Mostafa Imeni
Biodiversitat, Ecología, Technologia Ambiental i Alimentaria )BETA Tech Center(, (TECNIO Network), U Science Tech, University of Vic-Central University of Catalonia, Carrer de la Laura 13, 08500 Vic, Spain
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Saber Molaei
Saber Molaei
AJA University of Medical Sciences, Tehran, Iran
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Seyed Kazem Hosseini
Seyed Kazem Hosseini
Quality Control Manager of Iranian Tissue Bank Research & Preparation Center, Director of Stem Cells Preparation Unit, Tehran University of Medical Sciences, Tehran, Iran
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Zohreh Kalafi
Zohreh Kalafi
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
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Sara Sharifi Yazdi
Sara Sharifi Yazdi
School of Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran
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Hedroosha Molla Agha Mirzaei
Hedroosha Molla Agha Mirzaei
Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Published Date
- Oct 25, 2021
Amniotic Membrane Transplantation for Persistent Epithelial Defects and Ulceration due to Pseudomonas Keratitis in a Rabbit Model
Abstract
Purpose: The use of amniotic membrane has been suggested in the treatment ofinfectious keratitis for its intrinsic anti-infective properties probably mediated by its antiinflammatory effects. The aim of this study was to investigate the effect of amniotic membrane transplantation (AMT) along with ciprofloxacin to cure the primary stages of Pseudomonas keratitis.
Methods: In total, 28 rabbits were selected and divided in four groups as follows: group 1 as control, group 2 with amniotic membrane, group 3 with ciprofloxacin, and group 4 with amniotic membrane combined with ciprofloxacin. About 0.05 cc suspension of Pseudomonas aeruginosa, 27853 ATCC was injected into corneal stroma.
Results: The results showed groups of AMT, AMT + ciprofloxacin, and ciprofloxacin had 0% perforation while the control group had 85.6%. Average infiltration of 5.5 mm was observed in ciprofloxacin group, 5 mm in AMT + ciprofloxacin group, 24 mm in AMT group, and finally 23.75 mm for control. Amniotic membrane showed to be effective in prevention of cornea perforation as well as remission of Pseudomonas keratitis. There was no significant difference between ciprofloxacin groups in comparison with ciprofloxacin + AMT group. However, regarding the anti-inflammatory effect, the process of improvement of inflammation in ciprofloxacin + AMT group was faster.
Conclusion: Transplantation of amniotic membrane in the primary stages of Pseudomonas keratitis treatment remarkably prevents the disease and it can be used to control its process.
Keywords:
Ciprofloxacin, Human Amniotic Membrane, Keratitis, Pseudomonas aeruginosa, Rabbit
References
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7. Mifflin MD, Mortensen XM. Intraoperative optical pachymetry in photorefractive keratectomy. J Cart Refract Surg 2018;45:495–500.
8. Ehlke GL, Krueger RR. Laser vision correction in treating myopia. Asia-Pacific J Ophthalmol 2016;5:434–437.
9. Palamar M, Yaman B, Akalin T, Yagci A. Amniotic membrane transplantation in surgical treatment of conjunctival melanoma: long-term results. Turkish J Ophthalmol 2018;48:15–18.
10. Asoklis RS, Damijonaityte A, Butkiene L, Makselis A, Petroska D, Pajaujis M, et al. Ocular surface reconstruction using amniotic membrane following excision of conjunctival and limbal tumors. Eur J Ophthalmol 2011;21:552–558.
11. Kaufman SC, Jacobs DS, Lee WB, Deng SX, Rosenblatt MI, Shtein RM. Options and adjuvants in surgery for pterygium. Ophthalmology 2013;120:201–208.
12. Mitomycin C. Comparison of three methods for the treatment of pterygium: amniotic membrane graft, conjunctival autograft and conjunctival autograft plus. Int J Orbital Disord Oculoplastic Lacrimal Surg 2007;26:5–13.
13. Gündüz K, Uçakhan OO, Kanpolat A, Günalp I. Nonpreserved human amniotic membrane transplantation for conjunctival reconstruction after excision of extensive ocular surface neoplasia. Eye 2006;20:351–357.
14. Grewal DS, Mahmoud TH. Dehydrated allogenic human amniotic membrane graft for conjunctival surface reconstruction following removal of exposed scleral buckle. Ophthalmic Surg Lasers Imaging Retina 2016;47:948–951.
15. Tamhane A, Vajpayee RB, Biswas NR. Evaluation of amniotic membrane transplantation as an adjunct to medical therapy as compared with medical therapy alone in acute ocular burns. Ophthalmology 2005;112:1963–1969.
16. Pereira Gomes JA, dos Santos MS, Cunha MC, Mascaro VLD, de Nadai Barros J, Barbosa de Sousa L. Amniotic membrane transplantation for partial and total limbal stem cell deficiency secondary to chemical burn. Ophthalmology 2003;110:466–473.
17. Torabi T, Abroun S. Amniotic fluid, an effective factor for umbilical cord blood hematopoietic stem cells in cell Transfus Apher Sci 2019;58:169–173.
18. Tejwani S, Kolari RS, Sangwan VS, Rao GN. Role of amniotic membrane graft for ocular chemical and thermal injuries. Cornea 2007;26:21–26.
19. Nubile M, Carpineto P, Lanzini M, Ciancaglini M, Zuppardi E, Mastropasqua L. Multilayer amniotic membrane transplantation for bacterial keratitis with corneal perforation after hyperopic photorefractive keratectomy: case report and literature review. J Cataract Refract Surg 2007;33:1636–1640.
20. Gicquel J, Bejjani RA, Ellies P. Amniotic membrane transplantation in severe bacterial keratitis. Cornea 2007;26:27–33.
21. Heidarzadeh S, Ghasemian A, Kalafi Z, Nikkhahi F, Soltan MM. Antimicrobial effects of amniotic membrane on some bacterial strains. Immunopathol Persa 2018;4:1–5.
22. Mehdi M, Soltan-Dallal M, Nikkhahi F, Molaei S, Hosseini K, Kazem M, et al. Effect of Amniotic membrane combined with ciprofloxacin in curing the primary stages of pseudomonal keratit. J Med Bacteriol 2012;1:31–37.
23. Kim HK, Park HS. Fibrin glue-assisted augmented amniotic membrane transplantation for the treatment of large noninfectious corneal perforations. Cornea 2009;28:170– 176.
24. Chen HC, Tan HY, Hsiao CH, Huang SCM, Lin KK, Ma DHK. Amniotic membrane transplantation for persistent corneal ulcers and perforations in acute fungal keratitis. Cornea 2006;25:564–572.
25. Kim SJ, Song CH, Sung HJ, Yoo YD, Geum DH, Park SH, et al. Human placenta-derived feeders support prolonged undifferentiated propagation of a human embryonic stem cell line, SNUhES3: comparison with human bone marrowderived feeders. Stem Cells Dev 2007;16:421–428.
26. Miller DD, Simmons NL, Stewart MW. Recurrent corneal erosion: a comprehensive review. Clin Ophthalmol 2019;13:325–335.
27. Bouchard CS, John T. Amniotic membrane transplantation in the management of severe ocular surface disease: indications and outcomes. Ocul Surf 2004;2:201–211.
28. Dua HS, Gomes JAP, King AJ, Maharajan VS. The amniotic membrane in ophthalmology. Diagnostic Surg Tech 2004;49:51–77.
29. Dogan C, Sevki O, Akif A, Burak O. Efficacy of fixation of the amniotic membrane on a symblepharon ring with continuous suturing in acute ocular chemical burn patients. Int Ophthalmol 2019;39:2103–2109.
30. Hanada K, Shimazaki J, Shimmura S, Tsubota K. Multilayered amniotic membrane transplantation for severe ulceration of the cornea and sclera. Am J Ophthalmol 2001;131:324–331.
31. Jeoung J, Yoon Y, Lee J. The effect of amniotic membrane transplantation on the treatment of necrotizing scleritis after pterygium excision. J Korean Ophthalmol Soc 2004;45:1981–1988.
32. Azuara-blanco A, Pillai CT, Dua HS. Amniotic membrane transplantation for ocular surface reconstruction. Br J Ophthalmol 1999;83:399–402.
33. Sangwan VS, Burman S, Tejwani S, Mahesh SP, Murthy R. Amniotic membrane transplantation: a review of current indications in the management of ophthalmic disorders. Indian J Ophthalmol 2007;55:251–260.
34. Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian M, et al. Properties of the amniotic membrane for potential use in tissue engineering. Eur Cells Mater 2008;15:88–99.
35. King AE, Paltoo A, Kelly RW, Sallenave J, Bocking AD, Challis JRG. Expression of natural antimicrobials by human placenta and fetal membranes. Placenta 2007;28:161–169.
36. Nguyen Lunde N, Haugland M, Broe K, Larsen B, Damgaard I, Pettersen SJ, et al. Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions. Biochimie 2017;139:27–37.
37. Soltan-dallal MM, Kalafi Z, Rastegar-lari A, Hosseini SK, Rahimi-foroushani A. The effect of reduced bacterial dilution on human amniotic membrane antibacterial activity, in vitro. Zahedan J Res Med Sci 2013;15:6–8.
38. Quarello F, Forneris G, Borca M, Pozzato M. Do central venous catheters have advantages over arteriovenous fistulas or grafts? J Nephrol 2006;19:265–279.
39. Kowalewski M, Pawliszak W, Zaborowska K, Navarese EP, Szwed A. Gentamicin-collagen sponge reduces the risk of sternal wound infections after heart surgery: meta-analysis. J Thorac Cardiovasc Surg 2015;149:1631– 1640.e6.
40. Harron K, Mok Q, Hughes D, Muller-pebody B, Parslow R. Generalisability and cost-impact of antibiotic-impregnated central venous catheters for reducing risk of bloodstream infection in paediatric intensive care units. PLoS One 2016;11:e0151348.5.
2. Mastropasqua L, Massaro-giordano G, Nubile M, Sacchetti M. Understanding the pathogenesis of neurotrophic keratitis: the role of corneal nerves. Cell Physiol 2016;September:717–724.
3. Remigio L, Leonidas T. Accelerated collagen cross-linking in the management of advanced Acanthamoeba keratitis. J Arq Bras Oftalmol 2019;8:103–106.
4. Weng S, Jan R, Chang C, Wang J, Su S. Risk of band keratopathy in patients with end-stage renal disease. Sci Rep 2016;6:28675.
5. Stefan P, Sanziana I, Liliana V, Costin L, Vanessa P, Ciuluvica R. Pseudophakic bullous keratopathy. Rom J Ophthalmol 2017;61:90–94.
6. Tonti E. Different graft thicknesses after Descemet stripping endothelial keratoplasty for bullous keratopathy in the two eyes of the same patient. Int Med Case Rep J 2019:55–59.
7. Mifflin MD, Mortensen XM. Intraoperative optical pachymetry in photorefractive keratectomy. J Cart Refract Surg 2018;45:495–500.
8. Ehlke GL, Krueger RR. Laser vision correction in treating myopia. Asia-Pacific J Ophthalmol 2016;5:434–437.
9. Palamar M, Yaman B, Akalin T, Yagci A. Amniotic membrane transplantation in surgical treatment of conjunctival melanoma: long-term results. Turkish J Ophthalmol 2018;48:15–18.
10. Asoklis RS, Damijonaityte A, Butkiene L, Makselis A, Petroska D, Pajaujis M, et al. Ocular surface reconstruction using amniotic membrane following excision of conjunctival and limbal tumors. Eur J Ophthalmol 2011;21:552–558.
11. Kaufman SC, Jacobs DS, Lee WB, Deng SX, Rosenblatt MI, Shtein RM. Options and adjuvants in surgery for pterygium. Ophthalmology 2013;120:201–208.
12. Mitomycin C. Comparison of three methods for the treatment of pterygium: amniotic membrane graft, conjunctival autograft and conjunctival autograft plus. Int J Orbital Disord Oculoplastic Lacrimal Surg 2007;26:5–13.
13. Gündüz K, Uçakhan OO, Kanpolat A, Günalp I. Nonpreserved human amniotic membrane transplantation for conjunctival reconstruction after excision of extensive ocular surface neoplasia. Eye 2006;20:351–357.
14. Grewal DS, Mahmoud TH. Dehydrated allogenic human amniotic membrane graft for conjunctival surface reconstruction following removal of exposed scleral buckle. Ophthalmic Surg Lasers Imaging Retina 2016;47:948–951.
15. Tamhane A, Vajpayee RB, Biswas NR. Evaluation of amniotic membrane transplantation as an adjunct to medical therapy as compared with medical therapy alone in acute ocular burns. Ophthalmology 2005;112:1963–1969.
16. Pereira Gomes JA, dos Santos MS, Cunha MC, Mascaro VLD, de Nadai Barros J, Barbosa de Sousa L. Amniotic membrane transplantation for partial and total limbal stem cell deficiency secondary to chemical burn. Ophthalmology 2003;110:466–473.
17. Torabi T, Abroun S. Amniotic fluid, an effective factor for umbilical cord blood hematopoietic stem cells in cell Transfus Apher Sci 2019;58:169–173.
18. Tejwani S, Kolari RS, Sangwan VS, Rao GN. Role of amniotic membrane graft for ocular chemical and thermal injuries. Cornea 2007;26:21–26.
19. Nubile M, Carpineto P, Lanzini M, Ciancaglini M, Zuppardi E, Mastropasqua L. Multilayer amniotic membrane transplantation for bacterial keratitis with corneal perforation after hyperopic photorefractive keratectomy: case report and literature review. J Cataract Refract Surg 2007;33:1636–1640.
20. Gicquel J, Bejjani RA, Ellies P. Amniotic membrane transplantation in severe bacterial keratitis. Cornea 2007;26:27–33.
21. Heidarzadeh S, Ghasemian A, Kalafi Z, Nikkhahi F, Soltan MM. Antimicrobial effects of amniotic membrane on some bacterial strains. Immunopathol Persa 2018;4:1–5.
22. Mehdi M, Soltan-Dallal M, Nikkhahi F, Molaei S, Hosseini K, Kazem M, et al. Effect of Amniotic membrane combined with ciprofloxacin in curing the primary stages of pseudomonal keratit. J Med Bacteriol 2012;1:31–37.
23. Kim HK, Park HS. Fibrin glue-assisted augmented amniotic membrane transplantation for the treatment of large noninfectious corneal perforations. Cornea 2009;28:170– 176.
24. Chen HC, Tan HY, Hsiao CH, Huang SCM, Lin KK, Ma DHK. Amniotic membrane transplantation for persistent corneal ulcers and perforations in acute fungal keratitis. Cornea 2006;25:564–572.
25. Kim SJ, Song CH, Sung HJ, Yoo YD, Geum DH, Park SH, et al. Human placenta-derived feeders support prolonged undifferentiated propagation of a human embryonic stem cell line, SNUhES3: comparison with human bone marrowderived feeders. Stem Cells Dev 2007;16:421–428.
26. Miller DD, Simmons NL, Stewart MW. Recurrent corneal erosion: a comprehensive review. Clin Ophthalmol 2019;13:325–335.
27. Bouchard CS, John T. Amniotic membrane transplantation in the management of severe ocular surface disease: indications and outcomes. Ocul Surf 2004;2:201–211.
28. Dua HS, Gomes JAP, King AJ, Maharajan VS. The amniotic membrane in ophthalmology. Diagnostic Surg Tech 2004;49:51–77.
29. Dogan C, Sevki O, Akif A, Burak O. Efficacy of fixation of the amniotic membrane on a symblepharon ring with continuous suturing in acute ocular chemical burn patients. Int Ophthalmol 2019;39:2103–2109.
30. Hanada K, Shimazaki J, Shimmura S, Tsubota K. Multilayered amniotic membrane transplantation for severe ulceration of the cornea and sclera. Am J Ophthalmol 2001;131:324–331.
31. Jeoung J, Yoon Y, Lee J. The effect of amniotic membrane transplantation on the treatment of necrotizing scleritis after pterygium excision. J Korean Ophthalmol Soc 2004;45:1981–1988.
32. Azuara-blanco A, Pillai CT, Dua HS. Amniotic membrane transplantation for ocular surface reconstruction. Br J Ophthalmol 1999;83:399–402.
33. Sangwan VS, Burman S, Tejwani S, Mahesh SP, Murthy R. Amniotic membrane transplantation: a review of current indications in the management of ophthalmic disorders. Indian J Ophthalmol 2007;55:251–260.
34. Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian M, et al. Properties of the amniotic membrane for potential use in tissue engineering. Eur Cells Mater 2008;15:88–99.
35. King AE, Paltoo A, Kelly RW, Sallenave J, Bocking AD, Challis JRG. Expression of natural antimicrobials by human placenta and fetal membranes. Placenta 2007;28:161–169.
36. Nguyen Lunde N, Haugland M, Broe K, Larsen B, Damgaard I, Pettersen SJ, et al. Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions. Biochimie 2017;139:27–37.
37. Soltan-dallal MM, Kalafi Z, Rastegar-lari A, Hosseini SK, Rahimi-foroushani A. The effect of reduced bacterial dilution on human amniotic membrane antibacterial activity, in vitro. Zahedan J Res Med Sci 2013;15:6–8.
38. Quarello F, Forneris G, Borca M, Pozzato M. Do central venous catheters have advantages over arteriovenous fistulas or grafts? J Nephrol 2006;19:265–279.
39. Kowalewski M, Pawliszak W, Zaborowska K, Navarese EP, Szwed A. Gentamicin-collagen sponge reduces the risk of sternal wound infections after heart surgery: meta-analysis. J Thorac Cardiovasc Surg 2015;149:1631– 1640.e6.
40. Harron K, Mok Q, Hughes D, Muller-pebody B, Parslow R. Generalisability and cost-impact of antibiotic-impregnated central venous catheters for reducing risk of bloodstream infection in paediatric intensive care units. PLoS One 2016;11:e0151348.5.