Download fulltext HTML
1.
Rezaei Moghadam M, Jafarinasab M-R, Yousefi Z, Sanjari Moghaddam A, Memarzadeh H, Rezaei Kanavi M. Aloe Vera Gel-derived Eye Drops for Alkaline Corneal Injury in a Rabbit Model. JOVR [Internet]. 2020Feb.3 [cited 2024Apr.24];15(1):7–15. Available from: https://knepublishing.com/index.php/JOVR/article/view/5932

https://doi.org/10.18502/jovr.v15i1.5932

statistics

  • 1514 Abstract Views
  • 546 PDF Views
  • 0 HTML Views
  • Cited by 2 articles

authors

  • Mohsen Rezaei Moghadam

    Mohsen Rezaei Moghadam

    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    https://orcid.org/0000-0002-9725-2444
  • Mohammad-Reza Jafarinasab

    Mohammad-Reza Jafarinasab

    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Zahraa Yousefi

    Zahraa Yousefi

    Torfeh Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Ali Sanjari Moghaddam

    Ali Sanjari Moghaddam

    Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Hajar Memarzadeh

    Hajar Memarzadeh

    School of Traditional Medicine, Shahid Sadoughi University of Medical Sciences, Ardakan, Yazd, Iran
  • Mozhgan Rezaei Kanavi

    Mozhgan Rezaei Kanavi

    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Published Date

  • Feb 3, 2020
This journal is publishing all the articles under Creative Commons Attribution License CC BY 4.0.

Aloe Vera Gel-derived Eye Drops for Alkaline Corneal Injury in a Rabbit Model

Journal of Ophthalmic and Vision Research (JOVR) / January–March 2020, Volume 15, Issue 1 / Pages 7–15

Abstract

Purpose: To investigate the efficacy of topical Aloe Vera (AV) gel-derived eye drops on the healing of alkali-burned corneas in rabbits.


Methods: Thirty alkali-burned corneas of 30 New Zealand albino rabbits were categorized into three groups: AV treatment group that received AV gel-derived eye drops four times a day; medical therapy (MT) group that received conventional treatment; and the control group. Clinical examinations together with digital imaging of the corneas were performed on days 0, 1, 2, 4, and 7. The area of the corneal epithelial defect (CED) was measured using ImageJ software. After euthanizing the rabbits, the affected corneas were evaluated by histopathological examination. Finally, the clinical and histopathological results were compared among the groups.


Results: The CED area on days 2 and 7 was significantly less in the AV group than that in the MT group (P = 0.007 and P = 0.024, respectively) and the control group (P = 0.003 and P = 0.037, respectively). None of the cases developed hypersensitivity reactions, limbal ischemia, descemetocele, or corneal perforation during the study period. Based on histopathology, the AV group had notably less keratocyte loss than the MT group (P = 0.001) and the control group (P = 0.022). The inflammatory response after the alkali burn was higher in the AV group than that in the controls (P = 0.028).


Conclusion: Short-term topical AV treatment was effective in healing alkali-burned corneas and hastened corneal re-epithelialization as compared to MT; however, AV gel-derived eye drops did not reduce the inflammatory response.

Keywords:

Alkali Burn, Aloe Vera, Epithelialization, Rabbits

References
1. Eslani M, Baradaran-Rafii A, Movahedan A, Djalilian AR. The ocular surface chemical burns. J Ophthalmol 2014;2014:196827.

2. Haring RS, Sheffield ID, Channa R, Canner JK, Schneider EB. Epidemiologic Trends of Chemical Ocular Burns in the United States. JAMA Ophthalmol 2016;134:1119–1124.

3. Cejka C, Cejkova J. Oxidative stress to the cornea, changes in corneal optical properties, and advances in treatment of corneal oxidative injuries. Oxid Med Cell Longev 2015;2015:591530.

4. Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloe vera: a systematic review. J Tradit Complement Med 2015;5:21–26.

5. Habeeb F, Shakir E, Bradbury F, Cameron P, Taravati MR, Drummond AJ, et al. Screening methods used to determine the anti-microbial properties of Aloe vera inner gel. Methods 2007;42:315–320.

6. Ramachandra C, Rao PS. Processing of Aloe vera leaf gel: a review. Am J Agric Bio Sci 2008;3:502–510.

7. Atiba A, Wasfy T, Abdo W, Ghoneim A, Kamal T, Shukry M. Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats. Clin Ophthalmol 2015;9:2019–2026.

8. Green K, Tsai J, Luxenberg M. Effect of aloe vera on corneal epithelial wound healing. Cutan Ocul Toxicol 1996;15:301–304.

9. Crispin SM. Notes on veterinary ophthalmology. Oxford, UK: Blackwell Publishing; 2005:55e56.

10. Ralph RA. Chemical injuries of the eye. In: Tasman W, Jaeger EA, editors. Duane’s clinical ophthalmology. Philadelphia, PA: Lippincott Williams & Wilkins; 2004:1e24.

11. Rezaei Kanavi M, Sahebjam F, Tabeie F, Davari P, Samadian A, Yaseri M. Short-term effects of extremely low frequency pulsed electromagnetic field on corneas with alkaline burns in rabbits. Invest Ophthalmol Vis Sci 2012;53:7881–7888.

12. American Veterinary Medical Association. AVMA guidelines for the euthanasia of animals: 2013 edition; 2013.

13. Rezaei Kanavi M, Tabeie F, Sahebjam F, Poursani N, Jahanbakhsh N, Paymanpour P, et al. Short-term effects of extremely low-frequency pulsed electromagnetic field and pulsed low-level laser therapy on rabbit model of corneal alkali burn. Exp Eye Res 2016;145:216–223.

14. Li Y, Feng G, Yi Y, Lin J. [The experimental investigation of epithelial healing in rabbit central corneal alkali wounds]. Yan Ke Xue Bao 1999;15:74–77. 15. Salman IA, Gundogdu C. Epithelial healing in experimental corneal alkali wounds with nondiluted autologous serum eye drops. Cutan Ocul Toxicol 2010;29:116–121.

16. Chung JH, Kang YG, Kim HJ. Effect of 0.1% dexamethasone on epithelial healing in experimental corneal alkali wounds: morphological changes during the repair process. Graefes Arch Clin Exp Ophthalmol 1998;236:537–545.

17. Lee HS, Lee JH, Kim CE, Yang JW. Anti-neovascular effect of chondrocyte-derived extracellular matrix on corneal alkaline burns in rabbits. Graefes Arch Clin Exp Ophthalmol 2014;252:951–961.

18. Baradaran-Rafii A, Eslani M, Haq Z, Shirzadeh E, Huvard MJ, Djalilian AR. Current and upcoming therapies for ocular surface chemical injuries. Ocul Surf 2017;15:48–64.

19. Maddula S, Davis DK, Maddula S, Burrow MK, Ambati BK. Horizons in therapy for corneal angiogenesis. Ophthalmology 2011;118:591–599.

20. Ahlawat KS, Khatka BS. Processing, food applications and safety of aloe vera products: a review. J Food Sci Technol 2011:48:525–533.

21. Rezazadeh F, Moshaverinia M, Motamedifar M, Alyaseri M. Assessment of anti-HSV-1 activity of aloe vera gel extract: an in vitro study. J Dent (Shiraz) 2016;17:49–54.

22. Tyler V. Herbs of choice. In: The therapeutic use of phyto medicine. New York: Binghamton Pharmaceutical Products Press;1994:131–135.