Download fulltext HTML
1.
Javadi F, Kheiri B, Rezaeian Akbarzadeh A, Rezaei Kanavi M. Thickness Profile of Donated Corneas Preserved in Optisol-GS versus Sinasol: An Ex-vivo Study. JOVR [Internet]. 2023Nov.30 [cited 2024May13];18(4):379–385. Available from: https://knepublishing.com/index.php/JOVR/article/view/14546

https://doi.org/10.18502/jovr.v18i4.14546

statistics

  • 299 Abstract Views
  • 202 PDF Views
  • 0 HTML Views

authors

  • Fatemeh Javadi

    Fatemeh Javadi

    Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Bahareh Kheiri

    Bahareh Kheiri

    Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Amir Rezaeian Akbarzadeh

    Amir Rezaeian Akbarzadeh

    TiamPharma Labs Company, Tehran, Iran
  • Mozhgan Rezaei Kanavi

    Mozhgan Rezaei Kanavi

    Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Published Date

  • Nov 30, 2023
This journal is publishing all the articles under Creative Commons Attribution License CC BY 4.0.

Thickness Profile of Donated Corneas Preserved in Optisol-GS versus Sinasol: An Ex-vivo Study

Journal of Ophthalmic and Vision Research (JOVR) / Oct–Dec 2023, Volume 18, Issue 4 / Pages 379–385

Abstract

Purpose: This study aimed to compare the thickness profile and the endothelial cell density (ECD) of donated corneas maintained in Optisol-GS with those preserved in Sinasol over seven days.
Methods: Twenty paired donor corneas were received from the Central Eye Bank of Iran. After recording the osmolarity of each medium, one of each of the cornea pairs was preserved in either Optisol-GS or Sinasol media. Then, slit-lamp biomicroscopy and specular microscopic examinations were performed at the baseline and on day seven. Visante optical coherence tomography (V-OCT) was also performed at 1 hour (h), 24h, 72h, and one week post-preservation. The specular microscopic and V-OCT values were then compared between the two groups.
Results: The mean osmolarity of the Sinasol group was significantly less than the Optisol- GS group (296 vs. 366 mOsm/L, p = 0.0008). The mean central corneal thickness at the measurement points was comparable between the two groups. However, the increase of thickness one week post-preservation in the Sinasol group was remarkably lower than those in the Optisol-GS group (p = 0.019).
Conclusion: Corneal storage in Sinasol over seven days provides better and superior maintenance and preservation of corneal tissue deturgescence and a lower rate of ECD loss over Optisol-GS.

Keywords:

Optisol-GS, Sinasol, Corneal Thickness, Visante Optical Coherence Tomography

References
1. Basak S, Prajna NV. A Prospective, In Vitro, Randomized Study to Compare Two Media for Donor Corneal Storage. Cornea. 2016;35:1151–1155.

2. Lindstrom RL. Advances in corneal preservation. Trans Am Ophthalmol Soc. 1990;88:555–648.

3. Biswell R. Vaughan & Asbury’s General Ophthalmology. 18 ed. Riordan-Eva P, Cunningham ET, editors. United States of America; LANGE Clinical Medicine; 2011. Chapter 6, Cornea.

4. Wilson SE, Bourne WM. Corneal preservation. Surv Ophthalmol. 1989;33:237–259.

5. Patel D. I notes ophthalmology PG exam notes, cornea [Internet]. 1st ed. ’DB’ Da Books; 2017. p 27. Available from: https://books.google.ae/books/about/I_Notes_Cornea. html?id=MhtKDwAAQBAJ&redir_esc=y

6. Jeng BH. Preserving the cornea: corneal storage media. Curr Opin Ophthalmol. 2006;17:332–337.

7. Kanavi MR, Javadi MA, Chamani T, Fahim P, Javadi F. Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C. Cell Tissue Bank. 2015;16:243–247.

8. Nelson LR, Hodge DO, Bourne WM. In vitro comparison of Chen medium and Optisol-GS medium for human corneal storage. Cornea. 2000;19:782–787.

9. Pham C, Hellier E, Vo M, Szczotka-Flynn L. Donor endothelial specular image quality in Optisol GS and Life4 ˚C. Int J Eye Bank. 2013;1:1–8.

10. Parekh M, Salvalaio G, Ferrari S, Amoureux MC, Albrecht C, Fortier D, et al. A quantitative method to evaluate the donor corneal tissue quality used in a comparative study between two hypothermic preservation media. Cell Tissue Bank. 2014;15:543–554.

11. Javadi MA, Rezaeian Akbarzadeh A, Chamani T, Rezaei Kanavi M. Sinasol versus Optisol-GS for cold preservation of human cornea: a prospective ex vivo and clinical study. Cell Tissue Bank. 2021;22:563–574.

12. Kanavi MR, Nemati F, Chamani T, Kheiri B, Javadi MA. Measurements of donor endothelial keratoplasty lenticules prepared from fresh donated whole eyes by using ultrasound and optical coherence tomography. Cell Tissue Bank. 2017;18:99–104.

13. Rezaei Kanavi M, Chamani T, Kheiri B, Javadi MA. Preparation of endothelial keratoplasty lenticules with Gebauer SLc Original versus Moria CBm Carriazo- Barraquer and Moria One-Use Plus microkeratomes. Indian J Ophthalmol. 2020;68:762–768.

14. Tang M, Ward D, Ramos JL, Li Y, Schor P, Huang D. Measurements of microkeratome cuts in donor corneas with ultrasound and optical coherence tomography. Cornea. 2012;31:145–149.

15. Walkenbach RJ, Boney F, Ye GS. Corneal function after storage in dexsol or optisol. Invest Ophthalmol Vis Sci. 1992;33:2454–2458.

16. Armitage WJ. Preservation of Human Cornea. Transfus Med Hemother. 2011;38:143–147.

17. De Belder AN. Dextran. 2nd ed. Little Chalfont: Armersham Biosciences; 2003 p 12, 31.

18. Ho JW, Jung H, Chau M, Kuchenbecker JA, Banitt M. Comparisons of Cornea Cold, a New Corneal Storage Medium, and Optisol-GS. Cornea. 2020;39:1017-1019.

19. Gimenes I, Pintor AVB, da Silva Sardinha M, Marañón- Vásquez GA, Gonzalez MS, Presgrave OAF, et al. Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review. Applied Sciences.2022;12(14):7079.

20. Greenbaum A, Hasany SM, Rootman D. Optisol vs. Dexsol as storage media for preservation of human corneal epithelium. Eye 2004;18, 519-524.