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Goebel CP, Sheibani N, Song Y-S, S. Zaitoun I, Wang S, A. D. Potter H, M. Sorenson C. Adenosine Receptors Expression in Human Retina and Choroid with Age-related Macular Degeneration. JOVR [Internet]. 2023Feb.13 [cited 2024May2];18(1):51–59. Available from: https://knepublishing.com/index.php/JOVR/article/view/12725

https://doi.org/10.18502/jovr.v18i1.12725

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authors

  • Collin P. Goebel

    Collin P. Goebel

    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
  • Nader Sheibani

    Nader Sheibani

    PhD. Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, 1111 Highland Ave., 9453 WIMR, Madison, WI 53705-2275, USA.
  • Yong-Seok Song

    Yong-Seok Song

    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
  • Ismail S. Zaitoun

    Ismail S. Zaitoun

    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
  • Shoujian Wang

    Shoujian Wang

    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
  • Heather A. D. Potter

    Heather A. D. Potter

    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA
  • Christine M. Sorenson

    Christine M. Sorenson

    Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA

Published Date

  • Feb 13, 2023
This journal is publishing all the articles under Creative Commons Attribution License CC BY 4.0.

Adenosine Receptors Expression in Human Retina and Choroid with Age-related Macular Degeneration

Journal of Ophthalmic and Vision Research (JOVR) / Jan–Mar 2023, Volume 18, Issue 1 / Pages 51–59

Abstract

Purpose: Adenosine signaling modulates ocular inflammatory processes, and its antagonism mitigates neovascularization in both newborns and preclinical models of ocular neovascularization including age-related macular degeneration (AMD). The adenosine receptor expression patterns have not been well characterized in the human retina and choroid.
Methods: Here we examined the expression of adenosine receptor subtypes within the retina and choroid of human donor eyes with and without AMD. Antibodies specifically targeting adenosine receptor subtypes A1, A2A, A2B, and A3 were used to assess their expression patterns. Quantitative real-time PCR analysis was used to confirm gene expression of these receptors within the normal human retina and choroid.
Results: We found that all four receptor subtypes were expressed in several layers of the retina, and within the retinal pigment epithelium and choroid. The expression of A1 receptors was more prominent in the inner and outer plexiform layers, where microglia normally reside, and supported by RNA expression in the retina. A2A and A2B showed similar expression patterns with prominent expression in the vasculature and retinal pigment epithelium. No dramatic differences in expression of these receptors were observed in eyes from patients with dry or wet AMD compared to control, with the exception A3 receptors. Eyes with dry AMD lost expression of A3 in the photoreceptor outer segments compared with eyes from control or wet AMD.
Conclusion: The ocular presence of adenosine receptors is consistent with their proposed role in modulation of inflammation in both the retina and choroid, and their potential targeting for AMD treatment.

Keywords:

Caffeine, Choroid, inflammation, Neovascularization, Neurodegeneration, Retina

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