Published date: Mar 25 2025

Journal Title: West Kazakhstan Medical Journal

Issue title: West Kazakhstan Medical Journal: Volume 67 Issue 1

Pages: 16 - 23

DOI: 10.18502/wkmj.v67i1.18271

Nadiar M. MussinDepartment of General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe

Kulyash R. ZhilisbayevaDepartment of Languages, West Kazakhstan Marat Ospanov Medical University, Aktobe

Akmaral BaspakovaDepartment of Epidemiology, West Kazakhstan Marat Ospanov Medical University, Aktobe

Reza ShiraziDepartment of Anatomy, School of Biomedical Sciences, Medicine & Health, UNSW Sydney, Sydney

Seyyed Mojtaba MousaviDepartment of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City

Saltanat ZhumagaliyevaDepartment for Scientific Work, West Kazakhstan Marat Ospanov Medical University, Aktobe

Amin Tamadonamintamaddon@yahoo.comDepartment of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe

Abstract Exosomes are extracellular vesicles with significant potential in clinical applications, particularly in diagnostics and therapeutics. Effective exosome isolation is critical to ensuring their functionality and integrity, especially for clinical uses such as regenerative medicine and cancer therapy. This mini-review provides a comparative analysis of three primary exosome isolation methods: ultracentrifugation (UC), size exclusion chromatography (SEC), and microfluidic-based techniques. UC, a traditional and widely used method, is effective but lacks scalability and may compromise exosome integrity. SEC offers high purity and scalability, making it a favorable option for clinical-grade applications. Meanwhile, microfluidic-based methods provide high precision and rapid processing, with potential in diagnostic and point-of-care applications, though challenges in scalability limit their use in large-scale production. Overall, SEC shows the most promise for clinical exosome production, with microfluidic techniques offering specialized utility in diagnostics. This review underscores the importance of selecting an appropriate isolation technique to advance exosome-based clinical applications.

Keywords: exosomes, ultracentrifugation, size exclusion chromatography, microfluidics, clinical applications

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