TY - JOUR AU - Omid Banafshi AU - Sherko Nasseri AU - Leila Farhadi AU - Masoud Alasvand AU - Mohammad Bagher Khadem-Erfan AU - Javad Hosseini AU - Saber Miraki AU - Fardin Fathi PY - 2021/11/04 Y2 - 2024/03/28 TI - The effects of supplemented sericin on in vitro maturation and preimplantation development of mouse embryos: An experimental study JF - International Journal of Reproductive BioMedicine (IJRM) JA - IJRM VL - 19 IS - 10 SE - Articles DO - 10.18502/ijrm.v19i10.9824 UR - https://knepublishing.com/index.php/ijrm/article/view/9824 AB - Background: Mouse embryo culture condition is an essential part of transgenic, reproductive and developmental biology laboratories. Mouse embryonic culture media may have a high risk of serum contamination with pathogens. Objective: To investigate the effect of sericin as an embryo culture medium supplement on in vitro maturation (IVM), in vitro fertilization (IVF), and development of the preimplantation embryo in mice.Materials and Methods: The effects of sericin at three concentrations (subgroups) of 0.1%, 0.5%, and 1% as a medium supplement on IVM, IVF, and in vitro development of mouse embryos were separately investigated and compared with a sericin-free (control) group. The cumulative effect of the three concentrations was evaluated for IVM + in vitro development and IVF + in vitro development as follow-up groups.Results: In the IVM group, compared to the control group, the number of oocysts reaching the MII stage was significantly higher when 1% sericin was used (161/208 = 77.4%). No significant results were observed in the IVF and in vitro development groups with different concentrations of sericin compared to the control group. Among the follow-up groups, in the IVM + in vitro development group, the number of oocytes was higher after passing the IVM and IVF and reaching the blastocysts stage when 1% sericin was used, compared with other sericin subgroups. A significant difference was also noted when compared with the control group (p = 0.048). The IVF + in vitro development study group, on the other hand, did not show any significant relationship.Conclusion: It can be concluded that 1% sericin can be used as a supplement in mouse embryo cultures to improve the IVM rate. Also, based on the findings, sericin appears to be an effective supplement which can have a positive effect on the development of embryos derived from IVM.Key words: Sericin, In vitro maturation, In vitro fertilization, Preimplantation embryo, Culture medium, Mice. ER -