TY - JOUR AU - Parisa Azizi AU - Malek Soleimani Mehranjani PY - 2019/10/01 Y2 - 2024/03/29 TI - The effect of green tea extract on the sperm parameters and histological changes of testis in rats exposed to para-nonylphenol JF - International Journal of Reproductive BioMedicine (IJRM) JA - IJRM VL - 17 IS - 10 SE - Articles DO - 10.18502/ijrm.v17i10.5290 UR - https://knepublishing.com/index.php/ijrm/article/view/5290 AB - Background: Para-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals.Objective: The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats.Materials and Methods: 24 adult male Wistar rats (215 ± 20 gr) were randomly divided into four groups (n = 6/each) – including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE – and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated.Results: A significant decrease in the sperm number, motility, viability and morphology (p < 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p < 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p < 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE.Conclusion: It seems GTE can be used as a potent antioxidant in the case of p-NP toxication.Key words: Green tea extract, Para-nonylphenol, Sperm, Testis, Rat. ER -