@article{Solgi_Amiri_Soleimani Asl_Saidijam_Mirzaei Seresht_Artimani_2021, title={Antiapoptotic and antioxidative effects of cerium oxide nanoparticles on the testicular tissues of streptozotocin-induced diabetic rats: An experimental study}, volume={19}, url={https://knepublishing.com/index.php/ijrm/article/view/9465}, DOI={10.18502/ijrm.v19i7.9465}, abstractNote={<p><strong>Background:</strong> Cerium dioxide nanoparticles (CNPs) due to the antidiabetic and antioxidant activities are proposed for the treatment of oxidative stress-associated diseases.</p> <p><strong>Objective:</strong> To examine the impact of CNPs on hyperglycemia-induced apoptosis and oxidative stress in the testis of diabetic rats.</p> <p><strong>Materials and Methods:</strong> Twenty-four male rats were divided into four groups (n = 6/each) as diabetic rats, CNPs group, diabetic + CNPs rats, and controls. The control group was fed only mouse food and water. Rats became diabetic through receiving streptozotocin (STZ) 60 mg/kg. CNPs were given to the rats at a dose of 30 mg/kg daily for 2 wk. Malondialdehyde and total thiol group (TTG) levels were measured using spectrofluorometer. Expression of b-cell lymphoma protein 2-associated X protein (BAX) and b-cell lymphoma protein 2 (<em>Bcl-2</em>) were investigated using quantitative real-time polymerase chain reaction. Western blot analysis was used to examine caspase 3 protein levels.</p> <p><strong>Results:</strong> The content of malondialdehyde significantly increased in the STZ-diabetic rats, while TTG levels demonstrated a remarkable decrease. Caspase-3, <em>BAX</em>, and <em>BAX/Bcl-2</em> mRNA ratio raised significantly in the STZ-diabetic rats. On the other hand, <em>Bcl-2</em> mRNA levels reduced in the testis of diabetic rats (p = 0.006). Intervention with CNPs caused a substantial increase in the TTG levels, while the malondialdehyde contents, caspase-3, <em>BAX</em> levels, as well as <em>BAX/Bcl-2</em> mRNA ratio were considerably decreased following CNPs treatment. Administration of CNPs increased mRNA levels of <em>Bcl-2</em> (p &lt; 0.0001).</p> <p><strong>Conclusion:</strong> CNPs treatment attenuates testicular apoptosis and oxidative stress induced by diabetes. This nanoparticle might be suggested for the treatment of diabetes-associated reproductive disorders.</p> <p><em><strong>Key words:</strong> Apoptosis, Nanoceria, Diabetes, Oxidative stress, Testis.</em></p&gt;}, number={7}, journal={International Journal of Reproductive BioMedicine (IJRM)}, author={Solgi, Torab and Amiri, Iraj and Soleimani Asl, Sara and Saidijam, Massoud and Mirzaei Seresht, Banafsheh and Artimani, Tayebe}, year={2021}, month={Aug.}, pages={589–598} }