@article{Jalilvand_Salarinia_Namdar Ahmadabad_Safdari_2021, title={The expression of miR-17 and miR-29a in placenta-derived exosomes in LPS-induced abortion mice model: An experimental study}, volume={19}, url={https://knepublishing.com/index.php/ijrm/article/view/9252}, DOI={10.18502/ijrm.v19i5.9252}, abstractNote={<p><strong>Background:</strong> The expression pattern of microRNAs in placenta-derived exosomes plays a crucial role in the regulation of immune responses and inflammation at the fetal–maternal interface.</p> <p><strong>Objective:</strong> Considering the immunomodulatory properties of miR-17 and miR-29a, we determined their expression levels in placenta-derived exosomes in a lipopolysaccharide (LPS)-induced abortion mice model.</p> <p><strong>Materials and Methods:</strong> A total of 14 pregnant BALB/c mice, aged 6–8 wk, were randomly divided into two groups (n = 7/each) on the gestational day 11.5. While the mice in the experimental group were treated with LPS, those in the control group were treated with Phosphate buffered saline; 5 hr after the treatment, the placental cells were isolated and cultured for 48 hr. Then, the <em>cell culture supernatants were collected</em> and used for <em>isolation</em> of exosomes. The isolated exosomes were confirmed by western blot and scanning electron microscopy. The miRNAs were then extracted from exosomes, and cDNA synthesized. The expression levels of miR-17 and miR-29a were evaluated by quantitative real-time PCR analysis.</p> <p><strong>Results:</strong> Our results showed that the expression levels of miR-29a in placenta-derived exosomes obtained from the experimental group increased significantly compared to the control group. Also, the expression levels of miR-17 in the placenta-derived exosomes obtained from the experimental group were found to decrease; however, it did not show significant changes compared with the control group (p ˃ 0.05).</p> <p><strong>Conclusion:</strong> Inflammatory reactions at the fetal–maternal interface can alter miRNAs expression patterns in placenta-derived exosomes, especially miRNAs with immunomodulatory effects such as miR-29a.</p> <p><strong><em>Key words: </em></strong><em>Exosome, miR-17, miR-29a, Placenta, Inflammation.</em></p&gt;}, number={5}, journal={International Journal of Reproductive BioMedicine (IJRM)}, author={Jalilvand, Tahereh and Salarinia, Reza and Namdar Ahmadabad, Hasan and Safdari, Mohammadreza}, year={2021}, month={Jun.}, pages={433–440} }