@article{Sampannang_Arun_Burawat_Sukhorum_Iamsaard_2019, title={Expression of testicular phosphorylated proteins in types 1 and 2 diabetes mellitus in mice: An experimental study}, volume={17}, url={https://knepublishing.com/index.php/ijrm/article/view/4822}, DOI={10.18502/ijrm.v17i8.4822}, abstractNote={<p><strong>Background:</strong> Types 1 and 2 diabetes mellitus (DM) are known to be the cause of sub/infertility. However, the comparisons of potential markers in spermatogenesis and steroidogenesis in DM males have never been elucidated.</p> <p><strong>Objective:</strong> This study aimed to examine the expressions of tyrosine-phosphorylated and steroidogenic acute regulatory (StAR) proteins in testis of DM mice.</p> <p><strong>Materials and Methods:</strong> Fifty-six male C57BL/6 mice were divided into four groups (n = 14/ each): control of MLD-STZ (multiple low doses of streptozotocin), MLD-STZ, control of HFD-STZ (high-fat diet with STZ), and HFD-STZ. MLD-STZ mice (type 1 DM) were intraperitoneally (i.p.) injected with STZ at 40 mg/kg BW for five days. HFD-STZ mice (type 2 DM) received an HFD for 14 days and i.p.-induced by STZ at 85 mg/kg BW and fed with HFD. At the end of the experiment (days 36 and 72), the expressions of phosphorylated proteins and StAR were examined.</p> <p><strong>Results:</strong> Tyrosine phosphorylated proteins were localized in late spermatids, luminal fluid, and Leydig cells. The intensities of phosphorylated 110, 85, 72, 60, and 55 kDas were lower in the 36 day-DM mice. Although such intensities were present in both groups, only 85 kDa in the MLD-STZ mice was higher in HFD mice at 72 days. StAR expressions in both groups were decreased than that of the controls.</p> <p><strong>Conclusion:</strong> Decreased expressions of StAR and tyrosine-phosphorylated proteins may be directly involved in low testosterone levels and impaired spermatogenesis. These findings support the notion that both DM types play a role in male infertility.</p&gt;}, number={8}, journal={International Journal of Reproductive BioMedicine (IJRM)}, author={Sampannang, Apichakan and Arun, Supatcharee and Burawat, Jaturon and Sukhorum, Wannisa and Iamsaard, Sitthichai}, year={2019}, month={Sep.}, pages={567–576} }