@article{Watcho_Ngueyong_Deeh_Bonsou Fozin_Ngadjui_Wankeu-Nya_Kamtchouing_2022, title={Amaranthus hybridus (Amaranthaceae) prevents the detrimental effects of cyclophosphamide on ovarian function in Wistar rats: An experimental study}, volume={20}, url={https://knepublishing.com/index.php/ijrm/article/view/11754}, DOI={10.18502/ijrm.v20i8.11754}, abstractNote={<p><strong>Background:</strong> Cyclophosphamide (CP) is an anticancer agent, but its chronic administration induces ovarian toxicity</p> <p><strong>Objective:</strong> We evaluated the effects of aqueous extract (AE) and methanol extract (ME) of Amaranthus hybridus (A. hybridus) on CP-induced ovarian toxicity in rats.</p> <p><strong>Materials and Methods:</strong> 40 female Wistar rats (10 wk, 170-200 gr) were distributed into 8 groups (n = 5/each) as follows: 1) healthy control; 2) CP+distilled water (10 ml/kg/d); 3) CP+3%-tween 80 (10 mL/kg/d); 4) CP+clomiphene citrate (2 mg/kg/d); 5, 6) CP+AE of A. hybridus (55 and 110 mg/kg/d); and 7, 8) CP+ME of A. hybridus (55 and 110 mg/kg/d). After 28 days of treatment, estrus cyclicity, ovarian and uterine weights as well as estradiol levels and ovarian histology were determined.</p> <p><strong>Results:</strong> CP induced ovarian toxicity after 28 days of exposure. More specifically, CP disturbed the estrus cycle, decreased ovary and uterus weights (p = 0.04), and the 17-β estradiol level (p = 0.04), and induced severe ovarian damages. Remarkably, A. hybridus significantly increased (p = 0.03) the ovarian weight (AE and ME at all doses) and uterus weight (ME at 110 mg/kg/d), compared with the CP-treated rats. Moreover, the 17-β estradiol level was significantly elevated (p = 0.02) in rats given clomiphene citrate and A. hybridus (AE 110 mg/kg/d; ME 55 mg/kg/d). Finally, the ovaries of rats given plant extracts had many corpus luteum and normal follicles, and no cystic follicles.</p> <p><strong>Conclusion:</strong> A. hybridus prevented the detrimental effects of CP on ovarian function, which could support its traditional use as a fertility enhancer.</p> <p><strong>Key words:</strong> Cyclophosphamide, Amaranthus hybridus, Toxicity, Estradiol, Rat.</p&gt;}, number={8}, journal={International Journal of Reproductive BioMedicine (IJRM)}, author={Watcho, Pierre and Ngueyong, Prechmy Carole Nsamou and Deeh, Patrick Brice Defo and Bonsou Fozin, Georges Romeo and Ngadjui, Esther and Wankeu-Nya, Modeste and Kamtchouing, Pierre}, year={2022}, month={Sep.}, pages={651–662} }