https://doi.org/10.18502/ijrm.v19i10.9821
statistics
- 36 Abstract Views
- 14 PDF Views
- 0 HTML Views
authors
-
Razieh Doroudi
Razieh Doroudi
Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
-
Zohre Changizi
Zohre Changizi
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
-
Seyed Noureddin Nematollahi-Mahani
Seyed Noureddin Nematollahi-Mahani
Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Published Date
- Nov 4, 2021
Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study
Abstract
Background: Vitrification as the most efficient method of cryopreservation, enables successful storage of oocytes for couples who undergo specific procedures including surgery and chemotherapy. However, the efficacy of in vitro maturation (IVM) methods with vitrified germinal vesicle (GV) oocytes could be improved.
Objective: As melatonin and follicular fluid (FF) might enhance IVM conditions, we used these supplements to assess the maturation rate of vitrified GV oocytes and their artificial fertilization rate.
Materials and Methods: Four hundred mouse GV oocytes were harvested, vitrified, and assigned into control (C-Vit-GV) and treatment groups of melatonin (M-Vit-GV), human follicular fluid (HFF-Vit-GV), and a combination (M + HFF-Vit-GV). A non-vitrified group of GV oocytes (non-Vit-GV) and a group of in vivo matured metaphase II (VivoMII) oocytes served as control groups to evaluate the vitrification and IVM conditions, respectively. Maturation of GV oocytes to MII and further development to two-cell-stage embryos were determined in the different groups.
Results: Development to two-cell embryos was comparable between the Vivo-MII and non-Vit-GV groups. IVM and in vitro fertilization (IVF) results in the non-Vit-GV group were also comparable with the C-Vit-GV oocytes. In addition, the IVM and IVF outcomes were similar across the different treatment groups including the M-Vit-GV, HFF-Vit-GV, M + HFF-Vit-GV, and C-Vit-GV oocytes.
Conclusion: Employing an appropriate technique of vitrification followed by suitable IVM conditions can lead to reasonable IVF outcomes which may not benefit from extra supplementations. However, whether utilizing other supplementation formulas could improve the outcome requires further investigation.
Key words: Vitrification, Germinal vesicle, In vitro oocyte maturation, Melatonin, Follicular fluid.
References
[2] Hatirnaz S, Ata B, Hatirnaz ES, Dahan MH, Tannus S, Tan J, et al. Oocyte in vitro maturation: A sytematic review. Turk J Obstet Gynecol 2018; 15: 112–125.
[3] Sciorio R. Cryopreservation of human embryos and oocytes for fertility preservation in cancer and non cancer patients: A mini review. Gynecol Endocrinol 2020; 36: 381–388.
[4] Al-Hasani S, Ozmen B, Koutlaki N, Schoepper B, Diedrich K, Schultze-Mosgau A. Three years of routine vitrification of human zygotes: Is it still fair to advocate slow-rate freezing? Reprod BioMed Online 2007; 14: 288–293.
[5] Krisher RL. In vivo and in vitro environmental effects on mammalian oocyte quality. Annu Rev Anim Biosci 2013; 1: 393–417.
[6] Sutton ML, Gilchrist RB, Thompson JG. Effects of in-vivo and in-vitro environments on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity. Hum Reprod Update 2003; 9: 35–48.
[7] Acuna-Castroviejo D, Escames G, Venegas C, Díaz-Casado ME, Lima-Cabello E, López LC, et al. Extrapineal melatonin: Sources, regulation, and potential functions. Cell Mol Life Sci 2014; 71: 2997– 3025.
[8] Ishizuka B, Kuribayashi Y, Murai K, Amemiya A, Itoh MT. The effect of melatonin on in vitro fertilization and embryo development in mice. J Pineal Res 2000; 28: 48–51.
[9] Gao C, Han HB, Tian XZ, Tan DX, Wang L, Zhou GB, et al. Melatonin promotes embryonic development and reduces reactive oxygen species in vitrified mouse 2-cell embryos. J Pineal Res 2012; 52: 305– 311.
[10] Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, et al. Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 2008; 44: 280–287.
[11] El-Raey M, Geshi M, Somfai T, Kaneda M, Hirako M, Abdel-Ghaffar AE, et al. Evidence of melatonin synthesis in the cumulus oocyte complexes and its role in enhancing oocyte maturation in vitro in cattle. Mol Reprod Dev 2011; 78: 250–262.
[12] Takada L, Martins Junior A, Mingoti GZ, Balieiro JCC, Coelho LA. Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos. Sci Agric (Piracicaba, Braz) 2010; 67: 393–398.
[13] Farahavar A, Shahne AZ, Kohram H, Vahedi V. Effect of melatonin on in vitro maturation of bovine oocytes. Afr J Biotechnol 2010; 9: 2579–2583.
[14] Yazdanpanah F, Khalili MA, Eftekhar M, Karimi H. The effect of vitrification on maturation and viability capacities of immature human oocytes. Arch Gynecol Obstet 2013; 288: 439–444.
[15] Luna HS, Ferrari I, Rumpf R. Influence of stage of maturation of bovine oocytes at time of vitrification on the incidence of diploid metaphase II at completion of maturation. Anim Reprod Sci 2001; 68: 23–28.
[16] Fasano G, Demeestere I, Englert Y. In-vitro maturation of human oocytes: Before or after vitrification? J Assist Reprod Genet 2012; 29: 507–512.
[17] Annes K, Muller DB, Vilela JA, Valente RS, Caetano DP, Cibin FW, et al. Influence of follicle size on bovine oocyte lipid composition, follicular metabolic and stress markers, embryo development and blastocyst lipid content. Reprod Fertil Dev 2019; 31: 462–472.
[18] Sirard MA, Roy F, Patrick B, Mermillod P, Guilbault LA. Origin of the follicular fluid added to the media during bovine IVM influences embryonic development. Theriogenology 1995; 44: 85–94.
[19] Funahashi H, Day BN. Effects of different serum supplements in maturation medium on meiotic and cytoplasmic maturation of pig oocytes. Theriogenology 1993; 39: 965–973.
[20] Sun FJ, Holm P, Irvine B, Seamark RF. Effect of sheep and human follicular fluid on the maturation of sheep oocytes in vitro. Theriogenology 1994; 41: 981–988.
[21] Mendez M, Argudo D, Soria M, Galarza L, Perea F. [Effect of the addition of melatonin in the oocyte maturation and/or vitrification medium on in vitro production of bovine embryos]. Rev Inv Vet Peru 2020; 31: e17557. (in Spanish)
[22] Tian H, Qi Q, Yan F, Wang Ch, Hou F, Ren W, et al. Enhancing the developmental competence of prepubertal lamb oocytes by supplementing the in vitro maturation medium with sericin and the fibroblast growth factor 2-leukemia inhibitory factor-insulin-like growth factor 1 combination. Theriogenology 2020; 159: 13–19.
[23] Tamura H, Tanabe M, Jozaki M, Taketani T, Sugino N. Antioxidative action of melatonin and reproduction. Glycative Stress Res 2019; 6: 192–197.
[24] Gutierrez-Añez JC, Lucas-Hahn A, Hadeler KG, Aldag P, Niemann H. Melatonin enhances in vitro developmental competence of cumulus-oocyte complexes collected by ovum pick-up in prepubertal and adult dairy cattle. Theriogenology 2021; 161: 285–293.
[25] Tamura H, Nakamura Y, Korkmaz A, Manchester LC, Tan DX, Sugino N, et al. Melatonin and the ovary: Physiological and pathophysiological implications. Fertil Steril 2009; 92: 328–343.
[26] Bahadori MH, Ghasemian F, Ramezani M, Asgari Z. Melatonin effect during different maturation stages of oocyte and subsequent embryo development in mice. Iran J Reprod Med 2013; 11: 11–18.
[27] Succu S, Pasciu V, Manca ME, Chelucci S, Torres-Rovira L, Leoni GG, et al. Dose-dependent effect of melatonin on postwarming development of vitrified ovine embryos. Theriogenology 2014; 81: 1058– 1066.