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Salehpour, S., Kazemi, M., Hosseini, S., Hajizadeh, N., Karimi, B., Abbasi, H., Nemati, M., Esmaeili, S., & Ziaee, H. (2024). Drug-free in vitro activation and autologous transplantation in infertile women with diminished ovarian reserve: An experimental pilot study. International Journal of Reproductive BioMedicine (IJRM), 22(6), 425-. https://doi.org/10.18502/ijrm.v22i6.16792

https://doi.org/10.18502/ijrm.v22i6.16792

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authors

  • Saghar Salehpour

    Saghar Salehpour

    Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mahsa Kazemi

    Mahsa Kazemi

    Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Sedigheh Hosseini

    Sedigheh Hosseini

    Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Nazanin Hajizadeh

    Nazanin Hajizadeh

    Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Bahareh Karimi

    Bahareh Karimi

    Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Hajar Abbasi

    Hajar Abbasi

    Preventative Gynecology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mitra Nemati

    Mitra Nemati

    Preventative Gynecology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Samaneh Esmaeili

    Samaneh Esmaeili

    Preventative Gynecology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Hasti Ziaee

    Hasti Ziaee

    School of Medicine, University of Central Lancashire, Preston, United Kingdom.

Published Date

  • Jul 31, 2024

Drug-free in vitro activation and autologous transplantation in infertile women with diminished ovarian reserve: An experimental pilot study

International Journal of Reproductive BioMedicine (IJRM) / International Journal of Reproductive BioMedicine (IJRM): Volume 22, Issue No. 6 / Pages 425– 432

Abstract

Background: Poor ovarian response and diminished ovarian reserves (DOR) significantly contribute to female infertility. Previous attempts have been made to enhance follicular growth and improve pregnancy outcomes in these participants.


Objective: This study aimed to assess the efficacy of the in vitro drug-free activation technique of the ovarian reservation and in vitro fertilization stimulation cycle outcomes in DOR participants.


Materials and Methods: This pilot phase study investigated the impact of in vitro activation (IVA) on ovarian reservation and in vitro fertilization outcome in 10 infertile women with DOR from May to December 2023 at Taleghani Infertility Center, Tehran, Iran. Participants underwent general surgery and laparoscopy, involving the removal of a portion of one ovary, immediate transfer to the laboratory, dissection into small cubes, and subsequent re-implantation into the cases’s ovary. The primary outcomes, include the count of retrieved oocytes, the number of oocytes reaching metaphase, and the secondary outcomes were the quantity and the number of embryos transferred, implantation rate, and occurrence of clinical pregnancy.


Results: The study revealed a significant increase in the antral follicle count before and after IVA (p = 0.033). Before IVA, the median estradiol level was 93.5 (57.0), which reduced to 79.0 (35.0) after IVA, indicating a statistically significant difference. On average, 2.3 (0.8) oocytes were retrieved, among which 1.5 (0.7) were metaphase II oocytes. The observed pregnancy rate among the 2 cases was 22.2%.


Conclusion: The current study suggests that IVA may positively impact follicular growth and pregnancy outcomes among women with DOR.


Key words: In vitro fertilization, Autologous transplantation, Infertility, Ovarian reserve.

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