@article{Sawadogo_Diarra_BIsseye_Compaore_Djigma_Ouermi_Ouattara_Simpore_2017, title={Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso)}, volume={12}, url={https://knepublishing.com/index.php/SJMS/article/view/931}, DOI={10.18502/sjms.v12i3.931}, abstractNote={<p><strong>ABSTRACT:</strong></p><p><strong>Background</strong>: Bacteriological diagnosis of <em>Campylobacter </em>spp, <em>Salmonella </em>spp and <em>Shigella </em>spp<em> </em>could be necessary in the case of infectious gastroenteritis syndrome.</p><p>The objective of this study was to diagnose concomitantly the three enteropathogenic bacteria by multiplex Real-Time PCR in stool culture samples in Ouagadougou (Burkina Faso).</p><p><strong>Materials and Methods</strong>: The study was conducted from February 5th to March 9<sup>th</sup>, 2013. Two hundred stool samples were received during the study period. The bacteria were identified by bacterial culture following by multiplex Real-Time PCR.</p><p><strong>Results:</strong> <em>Shigella </em>spp and <em>Campylobacter </em>spp were sought by culture in all 200 samples. Enteropathogenic <em>E. coli</em> was sought only in 37 samples from all children under 2 years old. The bacterial culture was positive in 12 stool samples. <em>Shigella </em>spp and <em>Salmonella </em>spp. were isolated respectively in 5 (2.5%) and 3 samples (1.5%). Enteropathogenic <em>E. coli</em> was isolated in 10.8% (4/37) of the samples tested.</p><p>The multiplex real-time PCR identified bacteria in 20 patients, including 17 cases of <em>Shigella </em>spp., 1 case of <em>Salmonella </em>spp. and 2 cases of <em>Campylobacter </em>spp.</p><p><strong>Conclusions</strong>: This study has highlighted the low frequency of 3 sought bacterial genera in stool samples. It has also demonstrated a significant difference between the culture and the multiplex Real-Time PCR method in the diagnosis of <em>Shigella</em>.</p&gt;}, number={3}, journal={Sudan Journal of Medical Sciences (SJMS)}, author={Sawadogo, Salfo and Diarra, Birama and BIsseye, Cyrille and Compaore, Tegwindé Rebeca and Djigma, Florencia W. and Ouermi, Djénéba and Ouattara, Aboubakar S. and Simpore, Jacques}, year={2017}, month={Sep.}, pages={163-173} }