Comparison of Microscopic and PCR for Detection Giardia sp. in the Human Fecal Sample at Bedog Watershed, Sleman, DIY
Giardia sp. is a gastrointestinal protozoan that is common in mammals and causes giardiasis. Detection of parasitic infections in stool samples can use different methods such as identification of Giardia and trophozoite cysts using a light microscope (saline and iodine) and gene amplification gdh. The aim of this study was to compare the detection of direct microscopic Giardia and PCR in healthy people at risk for Giardiasis
in the Bedog watershed, Sleman, DIY. The results of the examination using positive Giardia samples from microscopy were obtained at 4% (4/10). While the PCR results are 7% (7/100). The microscopic method and PCR did not have a significant difference in PCR so that certain microscopic conditions can still be recommended as a basic method in detecting Giardia cysts and trophozoites. The sensitivity and specificity of the direct microscope were 96.9%, and 100%, respectively. The sensitivity and specificity of molecular analysis (PCR) were 97.14% and 100%, respectively. Although PCR detection is more specific than microscopic, in this case, the microscope method can still be used as an initial detection method. While the important advantages of PCR testing, its ability to directly distinguish between different Giardia genotypes, will help deal with cases of Giardiasis. The results of this study indicate that confirmation using the PCR technique can strengthen microscope detection.
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