Artificial Insemination on the Etawah Grade Goats Using Frozen Semen of Gembrong Goat
Abstract
One of the efforts to conserve Gembrong Goats is through a crossbreeding with Etawah Grade Goats uses the artificial insemination (AI). This aim of the study was to find the result of AI using the frozen semen of Gembrong Goat on the Etawa Grade does. Eight heads selected Etawah Grade does age above 4 yr, weighted 32 kg to 57 kg and BCS 3–4 were estrous synchronized using 0.5 mL pgf2α hormone (lutelyze®) injected in intramuscular. The estrous observation was done during 60 h after synchronized by the histology epithelial cells of the vagina and the visual sign of estrous. AI was conducted after the onset of estrous through intravaginal. Gestation determined by transrectal ultrasonography (USG) on 50 d after AI. Descriptive analysis was applied in
the study. Sperm quality of the frozen semen shows that a good condition with motility and viability is 80 % and 85 %. All Etawah Grade does show the onset of estrous based visual signs, such as agitated, flicked the tail, the vulva was swelling, reddish and slimy. The observations of epithelial cells of the vagina to the characterized phase of estrous cycles. The result of the AI was not showed gestation after USG test, maybe caused by
inseminator skills.
Keywords: Artificial insemination, Estrous synchronization, Etawah grade goat, Frozen semen, Gembrong goat.
References
[1] Pamungkas FA, Batubara A, Doloksaribu M, Sihite E. Petunjuk Teknis Potensi Beberapa Plasma Nutfah Kambing Lokal Indonesia. [Technical Guidance of Potential of Some Germplasm Goal Local Indonesia]. Pusat Penelitian dan Pengembangan Peternakan, Departemen Pertanian, Jakarta; 2009. p. 21–23. [in Bahasa Indonesia]. http://peternakan.litbang.pertanian.go.id/fullteks/booklet/ sgd_kambing_lokal_2009.pdf?secure=1
[2] Scherf BD. World Watch List for Domestic Animal Diversity. 3rd ed. Food and Agriculture Organization of the United Nations, Rome; 2000. p. 123 http://www.fao. org/docrep/009/x8750e/x8750e00.htm
[3] Budisatria IGS, Sidadolog JPH, Maharani D, Sumadi. Conserving endangered breed: Case study of Gembrong Goats. Proceeding of the 16th the AAAP Animal Science Congress Vol. 1 10–14 November 2014, Yogyakarta 2014;135–140. https://repository. ugm.ac.id/273107/1/16th%20AAAP_UGM.Widi.pdf
[4] Bintara S, Maharani D, Budisatria IGS, Sidadolog JHP, Sumadi, Elieser S, Batubara A. Sperm quality of Gembrong Goat in Bali, East Java and North Sumatra after extended with Citrate-egg yolk, Tris-egg yolk and Andromed®. Proceeding the 6th International Seminar on Tropical Animal Production, Yogyakarta 2015;546–549. https://journal. ugm.ac.id/istapproceeding/article/view/30713
[5] Hasinah H, Inounu I, Subandriyo. Indonesia effort to concerve Gembrong goats. IJSE 2015;8:8894 https://ejournal.undip.ac.id/index.php/ijse/article/view/8266
[6] Leboeuf, B, Restall B, Salomon S. Production and storage of goat sperma for artificial insemination. J Anim Reprod Sci., 2000;62:113–141. https://www.ncbi.nlm.nih.gov/ pubmed/10924822
[7] Doloksaribu M, Pamungkas FA, Nasution S, Mahmilia F. Inovasi teknologi inseminasi buatan secara intrauterine dengan menggunakan semen beku terhadap kebuntingan kambing. [Innovation of intrauterine-made insemination technology using frozen semen against goat pregnancy]. Prosiding seminar Nasional Teknologi Peternakan dan Veteriner, Bogor 2011;479–484. [in Bahasa Indonesia]. http://perpustakaan-puslitbangnak.blogspot.co.id/2015/01/prosidingseminar-nasional-teknologi_26.html
[8] Boume L.D. Theory and Practice of Histological Technique. 3rd ed. Churchill Livingston, Edinburgh; 1990. p. 85 https://trove.nla.gov.au/work/10963990
[9] Mc Donald LD. Veterinary Endocrinology and Reproduction. 4rd ed. Lea and Febiger, London; 1989. p. 243-247 https://books.google.co.id/books?id= WWpyvWwQLNYC&dq=Mcdonald+LD.+Veterinary+Endocrinology+and+
Reproduction+4th&hl=id&source=gbs_navlinks_s
[10] Bhattacharyya HK, Ganai NA, Khan HM Khan. Fertility of local goats of Kashmir using semen of Boer Bucks. Wudpecker J Agric Res 2012;1 346–348. https://www.researchgate.net/publication/ 236983837_Fertility_of_local_goats_of_kashmir_using_frozen_semen_Of_Boer_bucks
[11] Inounu I. Upaya meningkatkan keberhasilan inseminasi buatan pada ternak ruminansia kecil. [Efforts to Increase the Success Rate of Artificial Insemination on Small Ruminan]. Wartazoa 2014;24:201–209. [in Bahasa Indonesia]. http://dx.doi.org/10. 14334/wartazoa.v24i4.1091
[12] Tambing SN, Gazali M Purwanto B. Pemberdayaan Teknologi Inseminasi Buatan pada Ternak Kambing. [Efforts to Increase the Success Rate of Artificial Insemination on Small Ruminant]. Wartazoa 2001;11:1–9. [in Bahasa Indonesia]. http://peternakan. litbang.pertanian.go.id/fullteks/wartazoa/wazo244-5.pdf?secure=1