Hepatitis B virus has infected some 2 billion people in the world. Indonesia is the second largest in the South East Asian Region (SEAR) after Myanmar . According to Riskesdas results in 2013, the number of people who diagnose with symptoms doubled over 2007 data.
Hepatitis B virus is a significant cause of chronic hepatitis, cirrhosis, and hepatocellular carcinoma . The transmission is mainly through the mother to her baby at the time of parturition, as well as the transfer from person to person among children . Although they not infected during the perinatal period, children of HBV-infected mothers have a high risk of hepatitis B virus infection in the first five years of life . The effect of viral hepatitis in pregnant women increases the incidence of abortion, parts premiums, and bleeding. The risk to the fetus is premature, fetal death and transmission of viral hepatitis . This study aimed to determine the prevalence and proportion of pregnant women who are suffering from hepatitis B.
The source of population data was from the Central Bureau of Statistics of DKI Jakarta province. The object of research was all pregnant women of the first trimester to the third trimester of all Puskesmas in five areas of DKI Jakarta Province. Serum examination materials were laboratory tests for hepatitis B using Chemiluminescent Immunoassays (CLIA) method and using the Diasorin Liaison XL instrument. CLIA method was usually used for screening Hepatitis B virus because it can show higher sensitivity and specificity than previous methods. Also, CLIA methods can be used to measure HBs Ag titers in serum.
First of all, reagents containing magnetic beads coated with specific primary antibodies were mixed and incubated with serum. After incubation was complete, washing was done to remove unbound enzymes.
The next step was the addition of a secondary antibody in the form of analysis which was also coated by the same magnetic beads as in the primary antibody. Because tied by two antibodies then this technique was called the sandwich technique. The second antibodywas also coated by a stable enzyme and would catalyze the result of the reaction. The most widely used proteins for luminescent immunoassays were AP (Alkaline Phosphatase) and HRP (Horseradish Peroxidase). Enzymes linked to secondary antibodies that had interacted with the desired antigen would convert the substrate to produce a final product of a reaction emitting a light photon. Luminescence was used as an indicator to calculate the outcome of the response. The released photons would then detect by the instrument/tool. This reaction will measure in relative light units (RLUs / Relative light units) which were proportional to the number of analytes present in the sample.
3. Results and Discussions
Patients with Hepatitis B in pregnant women in DKI Jakarta 2015 were as many as three people per 100.000 population (2.76/100.000), with 95% confidence interval. This number increased compared to 2014, ie, two people per 100.000 population (1.66/100.000). The highest prevalence was in Central Jakarta (4.9/100.000), the lowest in South Jakarta (1.97/100.000). The incidence of hepatitis in all regions increased compared to 2014. The most significant increase occurred in West Jakarta (47.9%), and the lowest increase was in South Jakarta area (20.1 %). The proportion of pregnant women with Hepatitis B in 2015 was 2.12%, increase compared to the previous year which amounted to 1.98%. From the five areas of DKI Jakarta, the highest proportion occurred in North Jakarta at 2.57%, while the lowest was in South Jakarta with 1.65%
The prevalence of hepatitis B in pregnant women in 2015 in DKI Jakarta increased compared to 2014. The incidence of Hepatitis B in Central Jakarta was higher than in other regions. The most significant proportion of pregnant women who were positive for HBs Ag found in North Jakarta (table 1). One of the risk factors for the high prevalence of Hepatitis B was in areas with lower economic levels and the availability of health facilities. According to 2013 riskesdas data, based on the ownership index quintile, the smallest group occupied the highest prevalence of hepatitis compared to other groups. Chan and colleagues' studies showed an association between carrier Hepatitis B mothers and socioeconomic status such as family income . From the five areas in DKI Jakarta, the percentage of the poor was higher in North Jakarta (table 3). If the number of available government health facilities compared to the total population, the areas of West Jakarta and North Jakarta had the lowest percentage of the other regions (table 2). Central Jakarta and North Jakarta had fewer numbers than other areas (chart 1). Factors such as knowledge, behavior, environment, and others affected the spread and high incidence of Hepatitis B disease. Although infants born to hepatitis B mothers had vaccinated, the horizontal transmission in the household was an essential factor in the increase of hepatitis B infection . Increasing the prevalence of Hepatitis B patients in pregnant women in 2014 and 2015 will further increase the number of Hepatitis B patients in Indonesia, especially in DKI Jakarta in the future.
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Handling of hepatitis through community participation can be through promotive efforts such as personal hygiene and environment, cooking hygienic and sanitary foods, maintaining a diet to prevent obesity, avoiding risky sexual behavior, avoiding abuse of drugs and alcohol. Preventive measures that can be developed such as communication to receive immunization for infants, children, and pregnant women. Treatment and supervision of the patient to take medicine regularly and lifestyle clean and healthy. To overcome the outbreak, the community can play an active role to break the chain of transmission by maintaining the cleanliness of the environment, recommending that immediate treatment not to be a source of communication .
The prevalence of hepatitis B in DKI Jakarta in 2015 increased with the highest incidence was in Central Jakarta and North Jakarta. The two regions had a higher number of poor people compared to the other areas and had health facilities with fewer numbers than other areas.