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Nurhayati, A. P. D., Pratiwi, R., Wahyuono, S., & ., I. (2015). PROLIFERATION INHIBITORY ACTIVITY OF THE ACTIVE FRACTION MARINE SPONGE <i>Cinachyrella</i&gt; sp. AGAINST CELL LINE T47D. KnE Life Sciences, 2(1), 663-666. https://doi.org/10.18502/kls.v2i1.243

https://doi.org/10.18502/kls.v2i1.243

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authors

  • Awik Puji Dyah Nurhayati

    Awik Puji Dyah Nurhayati

    Biology Department, Mathematic and Natural Science Faculty, Sepuluh November Institute of Technology Surabaya
  • Rarastoeti Pratiwi

    Rarastoeti Pratiwi

    Biology Faculty ,Gadjah Mada University, Yogyakarta
  • Subagus Wahyuono

    Subagus Wahyuono

    Pharmacy Faculty , Gadjah Mada University, Yogyakarta
  • Istriyati .

    Istriyati .

    Biology Faculty ,Gadjah Mada University, Yogyakarta

Published Date

  • Sep 20, 2015

PROLIFERATION INHIBITORY ACTIVITY OF THE ACTIVE FRACTION MARINE SPONGE <i>Cinachyrella</i> sp. AGAINST CELL LINE T47D

KnE Life Sciences / International Conference on Biological Sciences (ICBS-2013) / Pages 663-666

Abstract

Marine sponges Cinachyrella sp. (Family:Tetillidae) in Kukup beach, Kemadang Village, Tanjungsari District, Gunung Kidul, DIY were producing diversity secondary metabolites such as polyketides, alkaloids, peptide and terpene. The purpose of this study was investigated proliferation inhibitory activity of active fraction Cinachyrella sp. against cell line T47D. Sponges samples were collected manually from rocky substrate at depth 0.5 m. The sponges was minced and extracted with 95% ethanol. The ethanol extract was partitioned sequentially with ethyl acetate. The extract ethyl acetate was fractionation with 4 organic solvent, in increasing order of polarity with vacuum liquid chromatography column (VLC) method. Doubling time method was applied to analyse the inhibition proliferative cell line T47D. Resulted showed ethyl acetate extract of Cinachyrella sp. were 12 fractions and all tested fraction obtained by thin layer chromatography (TLC). Fractions that have the same value Rf grouped together to obtain 6 fractions. The fraction number 5 exhibited proliferation inhibitory activity to cell line T47D. The Rf value of active fraction number 5 were 0.125; 0.25 and 0.437. The active fraction 5 than isolation by preparative thin-layer chromatography (PTLC) was 5 isolate fractions preparative. The isolate fractions preparative number 5 exhibited proliferation inhibitory activity against cell line T47D. Fraction which determined by cerium sulfate and results was expressed terpene and alkaloid.

Keywords: Cinachyrella sp., Doubling time method, proliferation inhibitory activity.

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