DOES <i>Beta vulgaris</i> L. HAVE LCYB GENE?
Abstract
Beta carotene is a pigment that generally shared by all plants. This pigment synthesis is catalized by lycopene beta cyclase enzime. This enzyme is encoded by the LCYB gene. This research aimed to obtain the sequence of LCYB gene isolated from beet (Beta vulgaris L.) leaves. Several experiments have been done to isolate the LCYB gene. In the first experiment, the gene primers are designed using Pick Primer software based on conserved region of Taraxacum officinale (accession no.AB247456.1) and Lilium lancifolium LCYB (accession no. GU471230.1).The forward primer is (5'-TGTCGTGGTGGATCTTGTGG-3') and the reverse is (5'-ACACCTGTTGAGCGACAGAC-3').At the first experiment, the gene target’s sequence was not obtained as the phylogenetic relationship between Beta vulgaris, Taraxacum officinale, and Lilium lancifolium were too far.In the second experiment, the primers are designed using LCYB gene in Arabidopsis thaliana (accsession no.NM_111858.2), with considering Arabidopsis thaliana as a plant model. The primers are (5’TGGGACAGCAGGAATGGTTC-3’) as the forward primer and (5’-GAGAAGAGCGACAACCCGAA-3’) the reverse. By using BLAST analysis in the second experiment, it suggests that the fragment amplified by forward primer has similarity with trnL gene.This study seems to amplify the trnL gene instead of LCYB gene. This facts leads to bigger question whether or not the Beta vulgaris L has the LCYB gene.
Keywords : Beta carotene, Lycopene Beta Cyclase, Beta vulgaris L, LCYB gene
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