INDIVIDUAL HUMAN SERA RESPONSE AGAINST PROTEIN EXTRACTS FROM SALIVARY GLAND OF <i>Aedes aegypti</i>
Abstract
The saliva of hematophagous arthropods contains a complex mixture of biologically active proteins. These proteins may modify hemostatic responses and induce both cellular immunity and the production of specific antibodies, and thus influence the transmission of its pathogens from arthropods vector to human host. Aedes aegypti is the main vector for transmission of dengue viruses into human. The objective of this study was to examine individual human sera response against protein extracts from salivary gland of Ae aegypti that mediate the infection of dengue viruses. We did a cross reaction test of human sera from healthy people in endemic and non-endemic area, and dengue patients againts SGE of Ae. aegypti to distinguish and to identify the immunogenic proteins using Western Blot Analysis. About 15 protein bands of SGE from Ae. aegypti ranging from 15 kDa up to 255 kDa were identified on 12% SDS-PAGE. Seven dominant bands were detected, i.e ~255, 56, 42, 31, 27, 26 and 15 kDa. Two immunogenic proteins, as represented by two bands, i.e. ~31 and 56 kDa were found only in samples from people who were previously exposed to mosquitoes bites, and not in people who had not been exposed. Therefore, these immunogenic salivary proteins may serve as indicators for the immune response in human against protein from salivary gland of Ae. aegypti.
Keywords: immunogenic proteins, salivary gland, Aedes aegypti
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