KnE Life Sciences | The Veterinary Medicine International Conference (VMIC) | pages: 579-587

DOI: 10.18502/kls.v3i6.1185

Potency of Bacillus cereus WPL 415 to Increase Crude Protein and Decrease Crude Fiber of Animal Feed Stuff

Widya Paramita Lokapirnasari
Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

Adriana Monica Sahidu
Department of Marine, Faculty of Fisheries and Marine, Airlangga University, Surabaya, Indonesia

Tri Nurhajati
Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

Koesnoto Soepranianondo
Department of Animal Husbandry, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

Andreas Berny Yulianto
Faculty of Veterinary Medicine,WijayaKusuma Surabaya University, Surabaya, Indonesia


This research aims to identify isolate as a probiotic candidate derived from liquor rumen of local beef cattle  and to know the ability of isolates as biofermentor on basal feed to the changes in the nutrient value. The selected samples were obtained from a slaughterhouse in Surabaya.  This study consisted of two stages.  The first stage was the identification of bacteria through the test of morphology, Gram staining, biochemical, resistance to acidity and 16S rDNA sequencing. The second stage was a test of the ability of the isolates on the nutrient of basal feeds by fermentation for three days in an aerobic condition. Based on the findings of the first phase, it has been identified that probiotic bacterium rods, motility positive, Gram-negative, have viability at pH 2 and pH 3 for 90 minutes and 24 hours and have the ability to ferment lactose, sucrose, galactose, ribose, cellobiose and xylose. Furthermore, based on test results of 16S rDNA sequencing, the probiotic bacterium was identified as Bacillus cereus WPL 415. Based on the research results at the second stage, Bacillus cereus WPL 415 at doses of 0.25% and 0.5% could improve the nutrient content of the basal feed. The results of the proximate analysis revealed that there was an increase in crude protein content of 6.78% until 8.12% compared to the control and was able to lower the crude fiber content of 15.19% and 17.40% compared to the control. Based on these results it can be concluded that Bacillus cereus WPL 415 from local beef cattle can be used as a probiotic candidates to improve the quality of animal feed.


Keywords: Bacillus cereus, probiotic, crude protein, crude fiber


R.Havenaar, B. Ten Brink, and J.H.Huis, Selection of strains for probiotic use. In Probiotics. (1992) 209-224. Springer Netherlands.

Y.Uyeno, S.Shigemori and T.Shimosato.J. Microbes and environments, 30(2)(2015)126-132.

C.Gil-Turnes, A.F.D.Santos, F.W.D. Cruz and A.V.Monteiro. CenBiot. Revista de microbiologia, 30(1) (1999)11-14.

R.Fuller, Probiotics. J. Appl. Bacteriol. SS, 1S-7S, 1986.

L.H.Duc, H.A.Hong, T.M.Barbosa, A.O.Henriques, and S.M.Cutting. Applied and environmental microbiology, 70(4)(2004)2161-2171.

J. Günther, and M, R.Jiménez. Revista de biología tropical, 52(4)(2004)937-943.

F.M.Ausubel, R. Brent, R.E.Kingston, D.D.Moore, J.G Seidman, J.A.Smith and K.Struhl, 2003. Current Protocols in Moleculer Biology. 2nd ed. John Willey & Sons, New York. .

G. D.Steel and J. H. Torrie.. Principles and procedure of statistics. New York: McGrow Hill Book Company.(1990).

M.NavinChandran,P.Iyapparaj,S.Moovendhan,R.Ramasubburayan, S.Prakash, G.Immanuel and A.Palavesam. Fish & shellfish immunology, 36(1)(2014)38-45

R.Havenaar, B. Ten Brink and J.H.Huis, 1992. Selection of strains for probiotic use. In Probiotics (pp. 209-224). Springer Netherlands.

L.H.Duc, H.A.Hong, T.M.Barbosa, A.O.Henriques and S.M.Cutting. Applied and environmental microbiology, 70(4)(2004)2161-2171.

L.M. Robson and G.H.Chambliss. Applied and Environmental Microbiology, 47(5) (1984)039-1046.

S.M.Aly, M.F.Mohamed and G.John, Effect of probiotics on the survival, growth and challenge infection in Tilapia nilotica (Oreochromis niloticus). Aquaculture research, 39(6):647-656(2008).

I. Murillo and L.Villamil, Journal of Aquaculture Research & Development. 2011.

L.R.Lynd, P.J. Weimer, W.H van Zyl and I.S.Pretorius. Microbiol. Mol. Biol. Rev.66(3) (2002)506-577.

N.W.Siti, I.G.N.G. Bidura and I.A.P.Utami. Journal of Biological and Chemical Research, 33(2016)214-225.

D.N. Kamra, Rumen microbial ecosystem. Current science, 89(1):24-135(2005).

I K.P. Nugraha, I.K.Sumadi, I.M. Mudita and I.W.Wirawan. e-jurnal Peternakan Tropika, 3(2)(2015)44-258.

I.Murillo and L.Villamil, . J. of Aquaculture Research & Development. 2011(2011).


  • Downloads 17
  • Views 126



ISSN: 2413-0877