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R, R. I., Diyatri, I., & Rahmawati, D. (2017). The Effectiveness of Antibiotics and Hematopoietic Stem Cell Treatment in Periodontitis Rat Model Toward TNF α Expression. KnE Life Sciences, 3(6), 513-519. https://doi.org/10.18502/kls.v3i6.1176

https://doi.org/10.18502/kls.v3i6.1176

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authors

  • Retno Indrawati R

    Retno Indrawati R

    Departement of Oral Biology, Faculty of Dentistry Airlangga University, Surabaya
  • Indeswati Diyatri

    Indeswati Diyatri

    Departement of Oral Biology, Faculty of Dentistry Airlangga University, Surabaya
  • Dwi Rahmawati

    Dwi Rahmawati

    Program Magister, Faculty of Dentistry Airlangga University, Surabaya Campus A Airlangga University, Jl. Mayjen. Prof. Dr. Moestopo No. 47 Surabaya

Published Date

  • Dec 3, 2017

The Effectiveness of Antibiotics and Hematopoietic Stem Cell Treatment in Periodontitis Rat Model Toward TNF α Expression

KnE Life Sciences / The Veterinary Medicine International Conference (VMIC) / Pages 513-519

Abstract

Periodontitis is a biofilm-induced chronic inflammatory. The current therapy of periodontitis is scaling root planning and curettage, and followed by administration of antibiotic such as combination of Amoxicillin and Clavulanic acid. Hematopoietic Stem Cell has ability to differentiate into blood cells which is capable of homing and regenerating itself. Research Purpose: The aim of this study was to prove antibiotic and Hematopoietic Stem Cell administration can reduce TNF α expression. Method: This research was divided into four different groups, P1 as negative control group, P2 as positive control group which inoculated with P.gingivalis 109 for three weeks as chronic periodontitis rat model and USP were administered, P3 as chronic periodontitis rat model received Hematopoietic Stem Cell injection into the tail vein of rat, P4 as chronic periodontitis rat models were given Amoxicillin and Clavulanic acid 250 mg/kg BW orally then followed by Hematopoietic Stem Cell 106 injection into the tail vein of rat. After two weeks rat were sacrified and immunohistochemically analysed for expression of TNF α. The data were analysed by using Non-Parametric Test. Result: TNF α expression of negative control group was different as compared to positive control group and treatment group which given Hematopoietic Stem Cell injection, but there is no difference between negative control group with treatment group which given  Amoxicillin and Clavulanic acid 250 mg/kg BW orally and followed by Hematopoietic Stem Cell injection. Clonclusion: The administration of Amoxicillin and Clavulanic acid 250 mg/kg BW and Hematopoietic Stem Cell can reduce TNF α expression on periodontitis rat model.

Keyword: Hematopoetic Stem Cell, TNF α, Chronic Periodontitis, Antibiotic

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