KnE Life Sciences | The Veterinary Medicine International Conference (VMIC) | pages: 497-512

DOI: 10.18502/kls.v3i6.1159

The Effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the Liver Structure of Iron overload of Rat (Rattus norvegicus)

Ratu Safitri
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km. 21, Jatinangor, Jawa Barat 45363, Phone +62227796412, Fax : +62227796412 Program Study Of Biotechnology, Post Graduate School Universitas Padjadjaran, Jl. Dipati Ukur no. 35, Bandung, Indonesia

Lelani Reniarti
Department of Pediatrics, Faculty of Medicine, Universitas Padjadjaran, Jl. Eijkman No. 38 Bandung 40162, Indonesia

Madihah Madihah
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km. 21, Jatinangor, Jawa Barat 45363, Indonesia

Lila Delia
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km. 21, Jatinangor, Jawa Barat 45363, Indonesia

Mas Rizky A.A Syamsunarno
Program Study Of Biotechnology, Post Graduate School Universitas Padjadjaran, Jl. Dipati Ukur no. 35, Bandung Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Padjadjaran, Jln. Raya Bandung-Sumedang KM.21, West Java, 45363, Indonesia

Ramdan Panigoro
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Padjadjaran, Jln. Raya Bandung-Sumedang KM.21, West Java, 45363, Indonesia

Abstract

This research was carried out to detect the effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the liver structure of iron overload rat (Rattus norvegicus).The method of experimental used Completely Random Design (CRD in triple repetition. The treatment had been carried out orally. Iron dextran with total dose of 1.5 g kg-1 of body were given to rat on the first, fourth, seventh, ten and thirteenth day. Sappan Wood Extract (Caesalpinia sappan)200 mg kg-1 bw, 400 mg kg-1 bw, Wheat grass extract 100 mg kg-1 bw and Vitamin E 60 mg kg-1 bw were given to rat everyday for 15 days. At the seventeenth, rat were killed and their liver were taken. The observed parameters are morphological abnormality including the colour, the contour, ratio between liver weight and body weight as well as histological destruction. The result showed iron dextran treatment was proved the abnormality on morphological and histological desruction. Futhermore, Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment can decrease the morphological abnormality and the liver histological destructionon of iron overload rat.

 

Keywords: Sappan Wood Extract, Wheat grass, Vitamin E, Iron, Morphological abnormality, Histological destruction

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