Oocyte Quality and Subsequent In Vitro Maturation of Sheep Oocyte-Cumulus Complex from Ovary with Presence and Absence of Corpus Luteum
Abstract
In vitro maturation is the crucial step for in vitro embryo production. It needs a large number of oocytes as source gamet cells recovered. The present study is aimed to assess the influence of corpus luteum on the average number oocytes harvested, COCs quality and subsequent maturation of immature oocytes recovered from sheep ovaries. Sheep ovaries were collected from local slaughterhouse and COCs were collected by using slicing method. Collected COCs were graded into three categories dependent upon cumulus cells surrounding them and the homogenous of cytoplasm. COCs were maturated in maturation media at 5% CO2 for 24 hours. Maturation of oocytes evaluated base on the expansion of cumulus cells and extrusion of the first polar body. There was significantly higher on average of COCs harvested from ovaries with corpus luteum compared without corpus luteum. The presence of Corpus luteum did not affect the COCs quality and ability to reach the maturation stage. However, there was a dramatic effect of cultured COCs quality on maturation rate both groups. Collectively, these results indicate that COCs quality is the main factor affecting the subsequent of oocytes matured in vitro.
Keywords: Corpus luteum; cumulus oocyte complex; in vitro maturation; maturation rate; ovaries
References
[1] Mapletoft, R. J.; Hasler, J. F., Assisted reproductive technologies in cattle: a review. Revue Scientifique et Technique-Office International des Epizooties 2005, 24 (1), 393
[2] Eppig, J. J., Coordination of nuclear and cytoplasmic oocyte maturation in eutherian mammals. Reproduction, Fertility and Development 1996, 8 (4), 485-489
[3] Tang, O. S.; Ng, E. H. Y.; Cheng, P. W.; Ho, P. C., Cortical vein thrombosis misinterpreted as intracranial haemorrhage in severe ovarian hyperstimulation syndrome: case report. Human Reproduction 2000, 15 (9), 1913-1916.
[4] Delvigne, A.; Rozenberg, S., Review of clinical course and treatment of ovarian hyperstimulation syndrome (OHSS). Human Reproduction Update 2003, 9 (1), 77-96 .
[5] de Souza-Fabjan, J. M. G.; Panneau, B.; Duffard, N.; Locatelli, Y.; De Figueiredo, J. R.; de Figueirêdo Freitas, V. J.; Mermillod, P., In vitro production of small ruminant embryos: Late improvements and further research. Theriogenology 2014, 81 (9), 1149-1162
[6] Contreras-Solis, I.; Diaz, T.; Lopez, G.; Caigua, A.; Lopez-Sebastian, A.; Gonzalez-Bulnes, A., Systemic and
6 Biotechnology Strengthen on Biomedical Science and Veterinary Medicine
intraovarian effects of corpus luteum on follicular dynamics during estrous cycle in hair breed sheep. Animal reproduction science 2008
[7] (a) Kumar, N.; Paramasivan, S.; Sood, P.; Singh, M., Micrometry of different category oocytes recovered from goat ovaries. Indian Journal of Animal Sciences (India) 2004; (b) Sahoo, L.; Singla, S. K., Effect of corpus luteum: quality and recovery rate of buffalo (Bubalus bubalis) oocytes. Turkish Journal of Veterinary and Animal Sciences 2013, 37 (4), 468-471; (c) Makwana, P. M.; Shah, R. G.; Singh, R. P.; Dhami, A. J., Influence of different categories of follicles and presence of cl on recovery rate, quality and quantity of buffalo oocytes. The Indian Journal of Animal Reproduction 2015, 33; (d) Nandi, S.; Chauhan, M. S.; Palta, P., Effect of a corpus luteum on the recovery and developmental potential of buffalo oocytes. Veterinary Record 2000, 147 (20), 580-581.
[8] Singh, S.; Dhanda, O. P.; Malik, R. K., Effect of the Presence of Corpus Luteum on Ooctye Recovery and Subsequent in vitro Maturation and Fertilization in Buffaloes. ASIAN AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES 2001, 14 (12), 1675-1677.
[9] Shabankareh, H. K.; Shahsavari, M. H.; Hajarian, H.; Moghaddam, G., In vitro developmental competence of bovine oocytes: Effect of corpus luteum and follicle size. Iranian journal of reproductive medicine 2015, 13 (10), 615.
[10] Asad, L.; Rahman, A.; Hossain, M. M.; Akter, M., Ovarian category, follicles and oocytes analysis of Goat ovaries in view of in vitro production of embryos.
[11] Tomac, J.; Cekinović, Đ.; Arapović, J., Biology of the corpus luteum. Periodicum biologorum 2011, 113 (1), 43-49.
[12] Hafez, E. S. E.; Hafez, B., Folliculogenesis, egg maturation, and ovulation. Reproduction in Farm Animals, 7th Edition 2000, 68-81.
[13] Kor, N. M., The effect of corpus luteum on hormonal composition of follicular fluid from different sized follicles and their relationship to serum concentrations in dairy cows. Asian Pacific journal of tropical medicine 2014, 7, S282-S288.
[14] Andersen, C. Y., Ovulation induction: Regulation of ovarian
androgen production: a key parameter to success during ovarian stimulation? Human Reproduction 1995, 10 (9), 2227-2233.
[15] Jurema, M. W.; Nogueira, D., In vitro maturation of human oocytes for assisted reproduction. Fertility and sterility 2006, 86 (5), 1277-1291.
[16] Eppig, J. J., Coordination of nuclear and cytoplasmic oocyte maturation in eutherian mammals. Reproduction, Fertility and Development 1996, 8 (4), 485-489.
[17] (a) Chauhan, M. S.; Katiyar, P. K.; Madan, M. L., In vitro production of blastocysts in goats, sheep and buffaloes. 2013;
(b) Warriach, H. M.; Chohan, K. R., Thickness of cumulus cell layer is a significant factor in meiotic competence of buffalo oocytes. Journal of veterinary science 2004, 5 (3), 247-251.
[18] Kelly, J. M.; Kleemann, D. O.; Rudiger, S. R.; Walker, S. K., Effects of grade of oocyte–cumulus complex and the interactions between grades on the production of blastocysts
in the cow, ewe and lamb. Reproduction in domestic animals 2007, 42 (6), 577-582.
[19] Wood, T. C.; Wildt, D. E., Effect of the quality of the cumulus–oocyte complex in the domestic cat on the ability of oocytes to mature, fertilize and develop into blastocysts in vitro. Journal of reproduction and fertility 1997, 110 (2), 355-360.
[20] Rodrigues, B. A.; Rodrigues, J. L., In vitro maturation of canine oocytes: a unique conundrum. Anim. Reprod 2010, 7, 3-15.
[21] Davachi, N. D.; Kohram, H.; Zainoaldini, S., Cumulus cell layers as a critical factor in meiotic competence and cumulus expansion of ovine oocytes. Small Ruminant Research 2012, 102 (1), 37-42 %@ 0921-4488.