KnE Life Sciences | The Veterinary Medicine International Conference (VMIC) | pages: 153-165

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Bitches have a single oestrous cycle during a breeding season so they are mono-oestrous and have spontaneous ovulation (Concannon, 2009). Pyometra (chronic purulent inflammation of the uterus) is a common reproductive disorder which affects nearly one fourth of all female dogs before they reach 10 years of age. The disease occurs mainly in middle-aged and older bitches and in the luteal phase of estrus cycle. Its pathogenesis is a consequence of repeated exposure to progesterone. Pyometra can also be caused by imbalance of hormones due to hormonal therapy to avoid pregnancy (Bigliardi et al., 2004). Cystic endometrial hyperplasia (CEH) often precedes the disease. CEH allows commensal bacteria originating from the vagina to proliferate in the uterus at the end of estrus. The progressive degenerative process of development of cystic endometrial hyperplasia is usually proposed as the initiating lesion for pyometra in bitches; this is mediated by progesterone and potentially aggravated by estrogens (Verstegen et al., 2008). There are two types of pyometra depending on the patency of the cervix, which are the open-cervix pyometra and closed-cervix pyometra. Open-cervix pyometra is identified with an obvious sign which is a sanguineous to mucopurulent discharge from the vagina. However, as closed-cervix pyometra lacks the easily recognized, early signs of a serious problem, namely the purulent vaginal discharge, bitches with closed-cervix pyometra is often quite ill at the time of diagnosis compared with dogs with open-cervix pyometra. The severity of illness at the time of examination depends to a large degree on the ability of the owner to recognize that a problem exist and seek veterinary care (Feldman and Nelson, 2004a). Bitches with pyometra exhibits multiple signs signifying reproductive and systemic disorders. Other than the obvious purulent discharge or vulva haemorrhage (especially for open-cervix pyometra), the clinical signs that can be seen includes dehydration, polydipsia, polyuria, lethargy, abdominal pain, anorexia, vomit or diarrhoea, fever or hypothermia, abnormal mucosal colour, tachycardia and increased heart rate are also the characteristic symptoms of pyometra (Smith, 2006). Pyometra in bitches is polysystemic diestrual disorder which if not treated can induce high mortality, because it may cause sepsis and endotoxemia. Bitches with pyometra requires rapid medical intervention to prevent death and to preserve fertility (Fransson and Ragle, 2003; Kutzler et al., 2014). So far, there has not been any research paper available about the analysis of pyometra in female dogs in Segar Veterinary Hospital, Kuala Lumpur, Malaysia. The data obtained is analysed using tree classification analysis and CATPCA (Principal Components Analysis for Categorical Data) analysis, where the cases of pyometra is analysed based on breed and age of dog. The results will be presented in a tree classification diagram and a joint plot of categorical points diagram. With the results, we will be able to learn the factors that affect the occurrence of pyometra in female dogs, such as the breed and age of the dog. Thus, preventive measures can be taken in order to lower the risk of pyometra cases in female dogs.

2. Materials and Methods

The secondary data was collected from Segar Veterinary Hospital, Kuala Lumpur, Malaysia and data analysis was done at Faculty of Veterinary Medicine, Universitas Airlangga. The period of data collected is from May 2012 to May 2016. The study case is pyometra in female dogs. The data about cases of pyometra occurred in Segar Veterinary Hospital, Kuala Lumpur, Malaysia, is collected from the ambulatoir or medical records. The ambulatoir contains data of the patients that supports the research, such as date of inspection, age, weight, diagnostic method of pyometra and the therapy given to the patient. The data that supports the research includes canine pyometra cases based on age and dog breed. The data collected from medical records or ambulatoir from Segar Veterinary Hospital, Kuala Lumpur, Malaysia, is further evaluated to find out if breed and age of the bitches are risk factors of canine pyometra. The data is also analysed to find out if there is any relationship between age and dog breed with pyometra cases using tree classification analysis and CATPCA (Principal Components Analysis for Categorical Data) analysis; the analysis is done by using program SPSS (Statistical Programs for Social Scientific) version 22.0. The output result obtained is the distribution frequency and is presented in the form of Tree Classification diagram and Joint Plot Category Points diagram.

3. Results and Discussion

According to the tree classification diagram in Figure 1, at an age of more than 5.5 years old, the following breeds' occurrence of open-cervix pyometra is almost 100% from 37 cases including Mongreal, German Shepard Dog, Mini Schnauzer, Silky Terrier, Toy Poodle, Beagle, Chow Chow, Golden Retriever, Rottweiler, Cocker Spaniel, White Terrier, Siberian Husky, and Pekingese; are significantly more inclined to having open-cervix pyometra rather than closed-cervix pyometra. However, dogs with the same breed aged 5.5 years old and younger have a percentage of 76.9% open-cervix pyometra from 10 cases, which is slightly lower compared to those in the higher age group.

This results are in accordance with previous studies of breed risk of pyometra in dogs where the breeds with increased risk are rough Collies, Rottweilers, Cavalier King Charles Spaniels, Golden Retrievers, Bernese Mountain Dogs and English Cocker Spaniels compared with baseline dogs. The present study further compared the increased risk of open-cervix pyometra in the breeds mentioned above, compared with the closed-cervix pyometra. The breeds German Shepard Dog, Mini Schnauzer, Silky Terrier, Toy Poodle, Beagle, Chow Chow which are at risk compared to previous studies possibly reflects that more of these breeds were included in the present study (Jitpean et al., 2012).

Figure 1

Tree Classification Diagram about Type of Pyometra Cases in Dogs Based On Age and Dog Breeds.

Table 1

Total number and percentage of Canine Pyometra cases based on Life stage.

Life stage Total
Year Puppies Adulthood Geriatric
Number (n) Percentage (%) Number (n) Percentage (%) Number (n) Percentage (%) Number (n) Percentage (%)
2012 0 0 4 5.00 6 7.50 10 12.50
2013 0 0 8 10.00 6 7.50 14 17.50
2014 0 0 9 11.25 10 12.50 19 23.75
2015 0 0 5 6.25 14 17.50 19 23.75
2016 0 0 4 5.00 14 17.50 18 22.50
Total 0 0 30 37.50 50 62.50 80 100.00

Table 1 show the number and percentage of canine pyometra based on the life stages that is adulthood and geriatric. Geriatric bitches had the highest number of pyometra cases at 50 cases and 62.5% while bitches in their adulthood which had pyometra are as much as 30 cases at 37.5%. In the present study, no pyometra cases from puppies were found.

These results are consistent with previous studies about the incidence of pyometra and their potential risk-factors which shows that the average age of the dogs affected by pyometra was frequently between 8-11 years, and the age of all cases was more than 4 years, probably accompanying the lowering of physiological resistance with aging (Fukuda, 2001; Hagman et al., 2011). Egenvall et al. (2001) reported no significant difference in the age at which dogs of different breeds presented with pyometra, however, the study only included dogs up to the age of 10 years, and so these figures may be skewed for breeds which have a higher mean age of pyometra presentation. The present study have cases where the dogs are as young as 2 years old, this could be explained as small breed dogs tend to achieve sexual maturity earlier than in larger dog breeds. Small breeds enter their first heat between 6 and 10 months of age, while some normal large-breed dogs do not begin their estrous cycle until 18 – 24 months of age (Feldman and Nelson, 2004b).

Table 2

Total number and percentage of Canine Pyometra cases based on type of breed.

Type of Breed Total
Year Small Medium Large
Number (n) Percentage (%) Number (n) Percentage (%) Number (n) Percentage (%) Number (n) Percentage (%)
2012 8 10.00 3 3.75 0 0 11 13.75
2013 10 12.50 3 3.75 1 1.25 14 17.50
2014 12 15.00 4 5.00 3 3.75 19 23.75
2015 15 18.75 3 3.75 0 0 18 22.50
2016 13 16.25 5 6.25 0 0 18 22.50
Total 58 72.50 18 22.50 4 5.00 80 100.00

Based on the type of breed categorised into small, medium and large breed, the small breed had the highest number of canine pyometra among the other breeds that is 58 cases at a percentage of 72.5%. This is followed by medium breed with 18 cases or 22.5% and large breed with 4 cases at 5%. A genetic predisposition for pyometra has not been demonstrated, however, a number of studies have described over-representation within certain large dog breeds, including rottweiler, golden retriever and Cavalier King Charles spaniel (Egenvall et al., 2001). A previous study in United Kingdom also showed an increased prevalence in larger dog breeds (bullmastiff, golden retriever, and dogue de Bordeaux) though it is thought that the population study could be skewed due to the preference of larger dog breeds (Gibson et al., 2013).

The pyometra population in this study may be skewed by an increased preference of small breed dogs as a companion animal in Kuala Lumpur, Malaysia due to lower maintenance cost, easier handled compared to larger dog breeds and many other factors. The study done by Egenvall et al. (2001) was done in a four season country where extreme differences in climate and temperatures could affect the cause of pyometra. Further investigation would be needed to assess whether this indicated a true breed predisposition.

Figure 2

CATPCA analysis uses optimal scaling to generalize principal components analysis to accommodate variables of mixed measurement levels. The point for geriatric dogs which are aged 7 years and above are far more nearer to the open-cervix pyometra point, whereas the point for adult dogs with a range from 2 to 6 years old is nearer to the closed-cervix pyometra point; indicating a relationship where geriatric dogs are more predisposed to have open-cervix pyometra and adult dogs have a higher possibility of having closed-cervix pyometra. There is also a correlation between the breeds of dogs based on body weight and the type of pyometra cases. Small breed dogs are seen to have a higher possibility of having open-cervix pyometra compared to medium or large breed dogs.

However, in a previous study by Gibson et al. (2013) there was a significant younger age at pyometra presentation from the larger dog breeds (bullmastiff and dogue de Boedeaux). In the previous studies, it was discussed that in some breeds the risk of pyometra may increase more and at a younger age than others, potentially as a result of a genetic predisposition for developing pyometra than other breeds so the possibilility of instituting breeding programmes for disease control might be considered (Egenvall et al., 2011).

The diagram also shows a correlation where geriatric dogs are more inclined to have open-cervix pyometra whereas closed-cervix pyometra is more frequently seen in adulthood bitches. This is slightly different with previous studies where closed-cervix pyometra is more common in old (>8 years), intact female dogs (Mylonakis et al., 2014). In another previous study, dogs with open-cervix the median age was 9.0 years and dogs with closed cervix pyometra the median age was 9.6 years, just slightly older than dogs affected with open cervix pyometra (Jitpean et al., 2017). The difference in results could be biased from the fact that closed-cervix pyometra is often left undiagnosed or undetected by dog owners due to the lack of the tell-tale clinical sign of pyometra that is vaginal discharge.

4. Conclusion

The risk factors of canine pyometra in Segar Veterinary Hospital, Kuala Lumpur, Malaysia in the period May 2012 to May 2016 that is at the age of more than 5.5 years old, the following breeds had 100% from 37 cases of open-cervix pyometra, including Mongreal, German Shepard Dog, Mini Schnauzer, Silky Terrier, Toy Poodle, Beagle, Chow Chow, Golden Retriever, Rottweiler, Cocker Spaniel, White Terrier, Siberian Husky, and Pekingese; are significantly more inclined to having open-cervix pyometra. Small breed and senior dogs are seen to have a higher possibility of having open-cervix pyometra while medium or large breed and adult dogs have a higher possibility of having closed-cervix pyometra. There should be further researches done to find out the effects of hormone therapy, frequency of births, and the bacteria present in uterus with pyometra.


With this opportunity, I would like to convey my heartfelt gratitude to all the people who have always been motivating and helping me throughout this research, especially to: Prof. Dr. Pudji Srianto, drh., M.Kes. as Dean of Faculty of Veterinary Medicine, Airlangga University and to all vice deans for giving me an opportunity to write and complete my thesis successfully.

Special thanks to Prof. Mas'ud Hariadi, drh., M.Phill, Ph.D. as my first advisor who is always patient and accommodating in guiding me to complete this thesis. Dr. Hardany Primarizky, drh., MVM. as my second advisor for her patience and thoroughness in reading and correcting the mistakes on my thesis, as well as giving advice that are always on point. Thank you to Prof. Dr. I Komang Wiarsa Sardjana, drh., as my first examiner Dr. Tita Damayanti L., drh., M.Sc, as my second examiner and Dr. Wiwik Misaco Yuniarti, drh., M.Kes., as my third examiner who has guided patiently and attentively, advise, counsel, which is very useful for myself to complete the writing successfully.

Thank you to my supervisor Prof. Hj. Romziah Sidik, drh., Ph.D who has always been giving me courage and support throughout all these semesters.

To my beloved parents Mr Ng Poh Boon and Mdm Lee Mun Hing and my siblings who has been always giving me strength and morale support to accomplish my ambition.

Thank you to the staff and doctors of Segar Veterinary Hospital, Kuala Lumpur, Malaysia who had given permission to carry out the research and for their assistance and patience throughout the research.

Lastly, I'm really thankful to my good friends Yugenthri Chandran, Ricadonna Raissa, Gowri Marriappan, Ahmad Akram, small class friends and also other friends for their help, courage and motivation they gave me.



Antonov, A.L., Atanasov, A.S., Fasulkov, I.R., Georgiev, P.I., Yotov, S., Karadaev, M. and Vasilev, N., 2015. Influence of some factors on the incidence of pyometra in the bitch. Bulgarian Journal of Veterinary Medicine, 18(4): 367-372.


Aqudelo, C.F. 2005. Cystic endometrial hyperplasia pyometra complex in cats. A review. The veterinary quarterly. 27(4): 173-182.


Arnold, S., I. Reichler and M. Hubler. 2006. Canine pyometra: new approaches to an old disease. World Congress WSAVA/FECAVA/CSAVA. 691-692.


Arora, N., Sandford, J., Browning, G.F., Sandy, J.R. and Wright, P.J., 2006. A model for cystic endometrial hyperplasia/pyometra complex in the bitch. Theriogenology, 66(6):1530-1536.


Bigliardi, E., Parmigiani, E., Cavirani, S., Luppi, A., Bonati, L. and Corradi, A., 2004. Ultrasonography and Cystic Hyperplasia–Pyometra Complex in the Bitch. Reproduction in Domestic Animals, 39: 136–140


Blendinger, K., 2007. Physiology and pathology of the estrous cycle of the bitch. In 56th Congresso Internazinale Multisala SCIVAC, Rimini. Proceedings of the 56th SCIVAC Congress 73-77.


Borresen, B. 1979. Pyometra in the dog—A pathophysiological investigation. II. Anamnestic, clinical and reproductive aspects. Nordic Vet. Med. 31: 251-257.


Borresen, B. 1980. Pyometra in the dog – a pathophysiological investigation. IV. Functional derangement of extragenital organs. Nordic Vet. Med. 32: 255-268.


Chastain, C.B., D. Panciera and C. Waters. 1999. Associations between age, parity, hormonal therapy and breed, and pyometra in Finnish dogs. Small Anim. Endocrinol. 9:8.


Concannon, P.W., Castracane, V.D., Temple, M. and Montanez, A., 2009. Endocrine control of ovarian function in dogs and other carnivores. Animal Reproduction, 6(1): 172-193.


Correa, J.E. 2002. Canine Breeding and Reproduction. Alabama A&M University. Alabama Cooperative Extension System.


De Bosschere, H., Ducatelle, R. and Tshamala, M., 2002. Is mechanically induced cystic endometrial hyperplasia (CEH) a suitable model for study of spontaneously occurring CEH in the uterus of the bitch. Reproduction in Domestic Animals, 37(3): 152-157.


Dominique, G., Josee and V. Jean-pierre. 2004. The Royal Canin Dog Encyclopedia. Aniwa S.A. French. Egenvall, A., Hagman, R., Bonnett, B.N., Hedhammar, A., Olson, P. and Lagerstedt, A.S., 2001. Breed risk of pyometra in insured dogs in Sweden. Journal of veterinary internal medicine, 15(6): 530-538.


Ewald, B.H., 1961. A survey of the cystic hyperplasia–pyometra complex in the bitch. Small Anim Clin 1:383– 386.


Fakuda, S., 2001. Incidence of pyometra in colony raised beagle dogs. Exp. Anim. 50: 325-328.


Feldman, E.C. and Nelson, R.W., 2004a. Cystic endometrial hyperplasia/ pyometra complex. In: Kersey R, editor. Canine and feline endocrinology and reproduction. WB Saunders Co. 852–867.


Feldman, E.C. and Nelson, R.W., 2004b. Ovarian Cycle and Vaginal Cytology. In: Kersey R, editor. Canine and Feline Endocrinology and Reproduction. WB Saunders Co. 526-546.


Foster R., 2016. Anatomy and Function of the Reproductive System in Dogs. Drs. Foster & Smith, Inc. Fransson, B., Lagerstedt, A.S., Hellmen, E. and Jonsson, P., 1997. Bacteriological findings, blood chemistry profile and plasma endotoxin levels in bitches with pyometra or other uterine diseases. Journal of Veterinary Medicine Series A, 44(1‐10) (Abstr.): 417-426.


Fransson, B.A. and Ragle, C.A., 2003. Canine pyometra: an update on pathogenesis and treatment. Compendium, 25(08): 602-612.


Fukuda, S., 2001. Incidence of pyometra in colony-raised beagle dogs. Experimental animals, 50(4): 325-329. Gibson, A., R. Dean, D. Yates and J. Stavisky. 2013. Retrospective study of pyometra at five RSPCA hospitals in the UK: 1728 cases from 2006 to 2011. Vet. Rec. 173(16): 396.


Gilbert, R.O., Nothling, J.O., Oettle, E.E. 1989. A retrospective study of 40 cases of canine pyometra-metritis treated with prostaglandin F-2 alpha and broad-spectrum antibacterial drugs. J Reprod Fertil Suppl 39:225–9.


Groppetti, D., Pecile, A., Arrighi, S., Di Giancamillo, A. and Cremonesi, F., 2010. Endometrial cytology and computerized morphometric analysis of epithelial nuclei: A useful tool for reproductive diagnosis in the bitch. Theriogenology, 73(7): 927-941.


Grundy, S.A., E. Feldman and A. Davidson. 2002. Evaluation of infertility in the bitch. Clin. Tech. Small Anim. Pract. 17: 108-115.


Hagman, R., Kindahl, H., Fransson, B.A., Bergstrِm, A., Holst, B.S. and Lagerstedt, A.S., 2006. Differentiation between pyometra and cystic endometrial hyperplasia/mucometra in bitches by prostaglandin F 2α metabolite analysis. Theriogenology, 66(2): 198-206.


Hagman, R., Lagerstedt, A.S., Hedhammar, Å. and Egenvall, A., 2011. A breed-matched case-control study of potential risk-factors for canine pyometra. Theriogenology, 75(7): 1251-1257.


Hardy, R.M., and Osborne, C.A. 1974. Canine pyometra: pathogenesis, physiology, diagnosis and treatment of uterine and extra-uterine lesions. J Am Anim Hosp Assoc. 10:245–68.


Hatmosrojo, R. and N.S. Budiana, 2008. Melatih Anjing Penjaga. Penebar Swadaya. Jakarta.


Jitpean, S., R. Hagman, B. Strom-Holst, O.V. Hoglund and A. Egenvall. 2012. Breed variance in the occurrence of pyometra and mammary tumours in Sweedish dogs. Dept. Clin. Sci. Fac. Vet. Med. Swed. Univ. Agric. Sci. SE-750 07 Uppsala. Sweden.


Jitpean, S., Ambrosen, A., Emanuelson, U. and Hagman, R., 2017. Closed cervix is associated with more severe illness in dogs with pyometra. BMC Veterinary Research, 13(1): 11.


Johnston, S.D., Kustritz, M.V.R., Olson, P.N.S. 2001. Disorders of the canine uterus and uterine tubes (oviducts). In: Kersey R, editor. Canine and feline theriogenology. WB Saunders Co. 206–24.


Kahn, C.M. and S. Line. 2005. The Merck Veterinary Manual. 9th ed. Merck & Co. Inc. USA.


Kaymaz, M., Baştan, A., Erünal, N., Aslan, S. and Findik, M., 1999. The use of laboratory findings in the diagnosis of CEH-pyometra complex in the bitch. Turkish Journal of Veterinary and Animal Sciences, 23(2): 127-134.


Kempisty, B., Bukowska, D., Wozna, M., Piotrowska, H., Jackowska, M., Zuraw, A., Ciesiolka, S., Antosik, P., Maryniak, H., Ociepa, E. and Porowski, S., 2013. Endometritis and pyometra in bitches: a review. Vet Med, 58(6): 289-297.


Kustritz, M.V.R., 2011. Clinical canine and feline reproduction: evidence-based answers. John Wiley & Sons, 149-153.


Kutzler, M.A., Krekeler, N. and Hollinshead, F. 2014. Pyometra. Small Animal Soft Tissue Surgery, 623-634. Meyers Wallen M.H., Goldschmidt Flickinger, G.L. 1986. Prostaglandin F2a treatment of canine pyometra. J Am Vet Med Assoc. 189:1557–1561.


Miklósi, Á., Kubinyi, E., Topál, J., Gácsi, M., Virányi, Z. and Csányi, V., 2003. A simple reason for a big difference: wolves do not look back at humans, but dogs do. Current Biology, 13(9): 763-766.


Miller, M.E., 1993. Anatomy of the Dog. W.B. Sounder Company. Philadelphia, 386-396.


Mylonakis, M.E., Koutinas, A.F., Baneth, G., Polizopoulou, Z., Yabsley, M.J., Varela, A.S., Tato, C.M., Dugan, V.G., Stalknecht, D.E., Little, S.E. and Davidson, W.R., 2014. Closed Cervix Pyometra in Canines. Indian Vet. J, 91(09): 78-80.


Nagasawa, M., Mogi, K. and Kikusui, T., 2009. Attachment between humans and dogs. Japanese Psychological Research, 51(3): 209-221.


Noakes, D., Parkinson, T.J., England, G.C. and Arthur, G.H., 2001. Arthur's veterinary reproduction and obstetrics. WB Saunders. 33-37, 650-655.


Olsen S. J. and Olsen J. W., 1977. The Chinese Wolf, Ancestor of New World Dogs. Science 197(4303): 533-535.


Pretzer, S.D., 2008a. Abnormalities of the Canine Estrous Cycle: A review. Article presented at the Canine Breeder's Symposium, St. Louis, Missouri 41-49


Pretzer, S.D., 2008b. Clinical presentation of canine pyometra and mucometra: a review. Theriogenology, 70(3):[359-363.]


Ramsingh, L., Rao, K.S. and Muralimohan, K., 2013. Clinical Management of Pyometra in Bitches. IOSR Journal of Pharmacy, 3(1): 13-14.

45, (2017). Dog Food by Size: Give Your Dog the Best. [12 January 2017]


Rynearson, E.K., 1978. Humans and pets and attachment. The British Journal of Psychiatry, 133(6): 550-555.


Sandholm, M., H. Vasenius and A.K. Kivisto. 1975. Pathogenesis of canine pyometra. JAVMA. 167: 1006-1010.


Savolainen, P., Zhang, Y.P., Luo, J., Lundeberg, J. and Leitner, T., 2002. Genetic evidence for an East Asian origin of domestic dogs. Science, 298(5598): 1610-1613.


Smith, B.J. and S. Mangkoewidjojo. 1988. Pemeliharaan, Pembiakan dan Penggunaan Hewan Cobaan di daerah Tropis. Universitas Indonesia Press. Jakarta.


Smith, F.O. 2006. Canine Pyometra. Theriogenology. 66: 610-612.


Stone, E.A., M.P. Littman, J.L. Robertson and K.C. Bovee. 1988. Renal dysfunction in dogs with pyometra with the antigestagen aglepristione. J. Vet. Med., series A. 50: 375-379.


Thrusfield, M., 2013. Veterinary epidemiology. Elsevier. 31-36.


Udell, M.A. and Wynne, C.D., 2008. A Review of Domestic Dogs' (Canis Familiaris) Human‐Like Behaviors: Or Why Behavior Analysts Should Stop Worrying And Love Their Dogs. Journal of the experimental analysis of behavior, 89(2): 247-261.


Verstegen, J., Dhaliwal, G. and Verstegen-Onclin, K., 2008. Mucometra, cystic endometrial hyperplasia, and pyometra in the bitch: advances in treatment and assessment of future reproductive success. Theriogenology, 70(3): 364-374.


Vilà, C., Savolainen, P., Maldonado, J.E., Amorim, I.R., Rice, J.E., Honeycutt, R.L., Crandall, K.A., Lundeberg, J. and Wayne, R.K., 1997. Multiple and ancient origins of the domestic dog. Science, 276(5319): 1687-1689.


Wheaton, L.G., Johnson, A.L., Parker, A.J., Kneller, S.K. 1989. Results and complications of surgical treatment of pyometra: a review of 80 cases. J Am Anim Hosp Assoc 25:563–8.



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