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Raharjo, I., & Prasetyo, A. A. (2015). A COMPLETE HIV-1 Env CODING SEQUENCES FROM HIV ISOLATED IN CENTRAL JAVA, INDONESIA. KnE Life Sciences, 2(1), 27-33. https://doi.org/10.18502/kls.v2i1.112

https://doi.org/10.18502/kls.v2i1.112

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authors

  • Irvan Raharjo

    Irvan Raharjo

    Biomedical Laboratory Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta
  • Afiono Agung Prasetyo

    Afiono Agung Prasetyo

    Biomedical Laboratory Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta Department of Microbiology Faculty of Medicine, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta Center of Biotechnology and Biodiversity Research and Development, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

Published Date

  • Sep 20, 2015

A COMPLETE HIV-1 Env CODING SEQUENCES FROM HIV ISOLATED IN CENTRAL JAVA, INDONESIA

KnE Life Sciences / International Conference on Biological Sciences (ICBS-2013) / Pages 27-33

Abstract

Background:The HIV envelope glycoprotein (env) plays a central role in viral transmission to target cells.The genetic diversity of env gene contributes a great mechanism on how HIV-1 could survive.Aims:To determine the genotype and genetic relationship of our isolate compared to the other HIV-1 isolates, especially from Indonesia based on the env gene. Also, for further use, the molecular properties of our gene had been analyzed. Methods: A complete coding sequence of HIV-1 env gene was cloned from 09IDSKA-6 (HIV isolated in Central Java, Indonesia), inserted into an Escherichia coli expression plasmid, and sequenced. The sequencing results of the env gene then subjected for virus subtyping and genotyping. The phylogenetic analysis was performed to know the relationship between the isolate with all HIV isolates in Indonesia based on the env gene.The sequencing results were also subjected for bioinformatics analysis.Results: Based on the molecular analysis of HIV-1 env complete coding sequences, the 09IDSKA-6 belong to HIV CRF01_AEwhich is quite common in Southeast Asia. The signal peptide, protein charge, transmembrane helix, antigenicity, hydrophobicity, secondary structure, and proteolytic cleavageof our gene also had been discussed.

Keywords: HIV-1, env gene, Indonesia. 

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