Magnetite Beads Binding to Human Serum Protein


Serum biomarker analysis of blood protein is often interfered with by the presence of dominant protein fractions such as albumin and globulin when analytical separation is performed. Magnetite solid phase extraction (MSPE) has been shown to be fast, effective and efficient to enable cleanup of proteins. Some studies have reported that magnetite adsorption to albumin is about 40-90% in a pure solution after several hours mixing and homogenizing. Few studies have examined the binding of magnetite to total protein in a human blood matrix. The magnetite coprecipitation method was performed in this study. Coprecipitation of magnetite was carried out by mixing 2:1 FeCl (III):(II) 0.01 M in an aqueous medium for 40 minutes at 90

[1] Zougman A, Wilson JP, Banks RE. Benchmarks a simple serum depletion method for proteomics analysis. Alexandre Zougman. 2020;62(2):149-152.

[2] Ghasemi E, Heydari A, Sillanpaa M. Superparamagnetic Fe3O4@EDTA nanoparticles as an efficient adsorbent for simultaneous removal of Ag(I), Hg(II), Mn(II), Zn(II), Pb(II) and Cd(II) from water and soil environmental samples. Microchemical Journal. 2017;131:51–6.

[3] Rahdar S, Rahdar A, Ahmadi S, Trant JF. Adsorption of bovine serum albumin (BSA) by bare magnetite nanoparticles with surface oxidative impurities that prevent aggregation. Canadian Journal of Chemistry. 97(8):577–83.

[4] Karimzadeh I, Aghazadeh M, Doroudi T, Superparamagnetic iron oxide (Fe3O4) nanoparticles coated with PEG/PEI for biomedical applications: A facile and scalable preparation route based on the cathodic electrochemical deposition method. Hindawi Advances in Physical Chemistry. 2017:0(0):1-7.

[5] Dinh VP, Tran NQ, Tranle NQ, et,al. Facile synthesis of FeFe2O4 magnetic nanomaterial for removing methylene blue from aqueous solution. 2019:29(6):648- 654.

[6] Sequeira AC. Electrochemical synthesis of iron oxide nanoparticles for biomedical application. Organic and Medical Chemistry International Journal. 2018;5(2):1-12.

[7] Antosova A, Gancar M, Zednarikova Z, et al. Surface-modified magnetite nanoparticles affect lysozyme amyloid fibrillization. Biochimica Biophysica Acta - General Subject. 2021;1865(9):1-11.

[8] Borah BM, Saha B, Dey SK, Das G. Surface-modification-directed controlled adsorption of serum albumin onto magnetite nanocuboids synthesized in a geldiffusion technique. Journal Colloid Interface Science. 2010;3491(1):114–21.

[9] Atta N, Galal A, Ahmed YM. Nano-magnetite/ionic liquid crystal modifiers of carbon nanotubes composite electrode for ultrasensitive determination of a new anti-hepatitis C drug in human serum. Journal Electroanalytical Chemistry. 2018;823(0):296–306.

[10] Deswal R, Narwal V, Suneja, Pundir CS. An ultrasensitive electrochemical immunosensor for detection of sex hormone binding globulin. Microchemistry Journal. 2019;149(2): 1-10.

[11] Mishra AK. Nanomedicine for drug delivery and therapeutics. Nanomedicine for Drug Delivery and Theraputic. 2013:0(0)20-55

[12] Dinh VP, Tranle NQ, Tran Q, et al. Facile synthesis of FeFe2O4 magnetic nanomaterial for removing methylene blue from aqueous solution. Progress Natural Science Materials International. 2019;29(6):648–54.

[13] Jansch M, Stumpf P, Grft C, Ruhl E, Muller RH. Adsorption kinetics of plasma proteins on ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. International Journal Pharmaceutics. 2012;428(1–2):125–33.

[14] Magdalena AG, Silba imb, Marques RFC, Filho PN, Jafelicci M. EDTA-functionalized Fe3O4 nanoparticles. Journal Physical Chemistry Solids. 2018;113:5–10.

[15] Schultz N, Metreveli G, Franzreb M, Frimmel FH, Syldatk C. Zeta potential measurement as a diagnostic tool in enzyme immobilisation. Colloids and Surfaces B Biointerfaces. 2008;66(1):39–44.

[16] Lahav J, Lawler J, Gimbrone MA. Thrombospondin interactions with fibronectin and fibrinogen: Mutual inhibition in binding. European Journal Biochemistry. 1984;1451:151–6.

[17] Khalil MI. Co-precipitation in aqueous solution synthesis of magnetite nanoparticles using iron(III) salts as precursors. Arabian Journal of Chemistry. 2015;8(2):279–84.

[18] Shah MT, Alveroglu E. Synthesis and characterization of magnetite nanoparticles having different cover layer and investigation of cover layer effect on the adsorption of lysozyme and bovine serum albumin. Materials Science and Engineering C. 2017;81:393–9.