Species of Fungi in the Root System of Woody Plants in Urban Plantations
Abstract
The features of the species composition of microscopic fungi in the root system and rhizosphere soil of woody plants in connection with the level of soil contamination have not been studied sufficiently. This article presents the results of studying the species composition of fungi in the root system and soil of three species of woody
plants (Acer negundo L., Acer platanoides L., and Betula pendula Roth.) growing in urban plantations of various ecological categories with different levels of heavy metal soil contamination. The study was carried out in a large industrial center of the Urals region, Izhevsk. When studying the species composition of fungi, microscopy
and molecular genetic analysis were used. Isolates of endotrophic fungi from the root system of plants were isolated, and systematic membership was determined by molecular genetic analysis. The results showed that in soils with a high level of contamination, the DNA of endotrophic mycorrhiza-forming fungi was found in the
roots of woody plants in a good living state.
Keywords: fungi, heavy metals, plantations, urban environment, resistance
References
[1] Fazel, R. S. A., Ian, J. A., Mwinyikione, M., et al. (2011). Effect of superphosphate and arbuscular mycorrhizal fungus glomus mosseae on phosphorus and arsenic uptake in lentil (Lens culinaris L.). Water, Air and Soil Pollution, vol. 221, pp. 169–182.
[2] Sun, Q., Dai, S., Zhang, C., et al. (2012). Mechanisms of mycorrhizal fungi in promoting nitrogen uptake and utilization by plants: A review. Chinese Journal of Ecology, vol. 31, no. 5, pp. 1302–1310.
[3] Casieri, L., AitLahmidi, N., Doidy, J., et al. (2013). Biotrophic Transportome in Mutualistic Plant-Fungal Interactions. Mycorrhiza, vol. 23, pp. 597–625.
[4] Taffouo, V. D., Ngwene, B., Akoa, A., et al. (2014). Influence of phosphorus application and arbuscular mycorrhizal inoculation on growth, foliar nitrogen mobilization, and phosphorus partitioning in cowpea plants. Mycorrhiza, vol. 24, no. 5, pp. 361–368.
[5] Segue, A., Cumming, J. R., Klugh-Stewart, K., et al. (2013). The role of arbuscular mycorrhizas in decreasing aluminium phytotoxicity in acidic soils. Mycorrhiza, vol. 23, pp. 167–183.
[6] Rouphaela, Y., Franken, P., Schneider, C., et al. (2015). Arbuscular mycorrhizal fungi act as biostimulants in horticultural crops. Scientia Horticulturae, vol. 196, pp. 91–108.
[7] Shabani, L., Sabzalian, M. R., and Mostafavi, S. (2016). Arbuscular mycorrhiza affects nickel translocation and expression of ABC transporter and metallothionein genes in Festuca arundinacea. Mycorrhiza, vol. 26, pp. 67–76.
[8] Wang, F., Liu, X., Shi, Z., et al. (2016). Arbuscular mycorrhizae alleviate negative effects of zinc oxide nanoparticle and zinc accumulation in maize plants – A soil microcosm experiment. Chemosphere, vol. 147, pp. 88–97.
[9] Wua, S., Zhanga, X., Chena, B., et al. (2016). Chromium immobilization by extraradical mycelium of arbuscular mycorrhiza contributes to plant chromium tolerance. Environmental and Experimental Botany, vol. 122, pp. 10–18.
[10] Bukharina, I. L., Zhuravleva, A. N., and Bolyshova, O. G. (2012). Urban Plantations: Ecological Aspects. Izhevsk: Udmurt University.
[11] Andrade-Linares, D. R., Grosch, R., and Franken, P. (2012). Screening of tomato entophytic fungi for potential biological agents. IOBC-WPRS Bulletin, vol. 83, pp. 69– 73.
[12] The list of annotated sequences presented in EMBL, http://www.ebi.ac.uk/
[13] NSBI databases, http://www.ncbi.nlm.nih.gov/
[14] Palmero, D., de Cara, M., Iglesias, C., et al. (2011). Comparative study of the pathogenicity of seabed isolates of Fusarium equiseti and the effect of the composition of the mineral salt medium and temperature on mycelial growth. Brazilian Journal of Microbiology, vol. 4, no. 3, pp. 948–953.
[15] Elsharkawy, M., Shimizu, M., Takahashi, H., et al. (2012). The plant growthpromoting fungus Fusarium equiseti and the arbuscular mycorrhizal fungus glomus mosseae induce systemic resistance against cucumber mosaic virus in cucumber plants. Plant Soil, vol. 361, pp. 397–409.
[16] Horinouchi, H., Watanabe, H., Taguchi, Y., et al. (2011). Fusarium equiseti GF191 as an effective biocontrol agent against Fusarium Crown and root rot of tomato in rock wool systems. BioControl, vol. 56, pp. 915–923.
[17] Bukharina, I. L. and Islamova, N. A. (2016). Investigation of the limits of stability of microscopic fungi and the formation of a collection of promising isolates, in Proceedings of the Annual Meeting of the Society of Plant Physiologists of Russia. St Petersburg: Publishing House of St Petersburg University.