Download fulltext HTML
Nurmamulyosari, L. D., Hariri, M. R., Gofur, A., Listyorini, D., Susanto, H., & Handaya, A. Y. (2015). EFFECT of <i>Aloe vera</i> EXTRACT TO THE INSULIN-LIKE GROWTH FACTOR-1 (IGF-1) LEVELS FROM VISCERAL FAT TISSUE IN <i>Rattus norvegicus</i&gt; WISTAR DIABETES MELLITUS. KnE Life Sciences, 2(1), 174-179. https://doi.org/10.18502/kls.v2i1.138

https://doi.org/10.18502/kls.v2i1.138

statistics

  • 352 Abstract Views
  • 216 PDF Views
  • 0 HTML Views

authors

  • Lulut Dwi Nurmamulyosari

    Lulut Dwi Nurmamulyosari

    Postgraduate Student of Biology Department, Postgraduate Programme Universitas Brawijaya
  • Muhammad Rifqi Hariri

    Muhammad Rifqi Hariri

    Postgraduate Student of Biology Department, Faculty of Mathematics and Natural Sciences Institut Pertanian Bogor
  • Abdul Gofur

    Abdul Gofur

    Biology Department, Faculty of Mathematics and Natural Sciences Universitas Negeri Malang
  • Dwi Listyorini

    Dwi Listyorini

    Biology Department, Faculty of Mathematics and Natural Sciences Universitas Negeri Malang
  • Hendra Susanto

    Hendra Susanto

    Biology Department, Faculty of Mathematics and Natural Sciences Universitas Negeri Malang
  • Adeodatus Yudha Handaya

    Adeodatus Yudha Handaya

    Faculty of Medicine Universitas Brawijaya

Published Date

  • Sep 20, 2015

EFFECT of <i>Aloe vera</i> EXTRACT TO THE INSULIN-LIKE GROWTH FACTOR-1 (IGF-1) LEVELS FROM VISCERAL FAT TISSUE IN <i>Rattus norvegicus</i> WISTAR DIABETES MELLITUS

KnE Life Sciences / International Conference on Biological Sciences (ICBS-2013) / Pages 174-179

Abstract

Diabetes mellitus is a metabolic disorder indicated by hyperglycaemia due to lack of insulin production, insulin activity, or both. It causes oxidative stress that may damage the receptor of Growth Hormone Releasing Hormone (GHRH) in the pituitary. As the result, Growth Hormone (GH) and Insulin-like Growth Factor-1 (IGF­1) production are decreased and glucose uptakes to the cell is inhibited. Aloe vera has a high antioxidant activity. It is possible to counter oxidative stress in unregulated the production of IGF-1. This study aimed to determine the effect of A. vera extracts to the level of IGF-1 from visceral fat tissue Rattus norvegicus wistar diabetes mellitus. This study used 24 R. norvegicus wistar male. R. norvegicus STZ-induced diabetes mellitus
R. norvegicus were grouped into positive control (C+), 30 mg/day, 60 mg/day, 120 mg/day. Fasting blood glucose test was performed to determine the successful induction of diabetes mellitus (DM) three days after induced. Non-DM rats (NDM) were grouped into negative control (C-), 30 mg/day, 60 mg/day, and 120 mg/ day. Each group was consisting of three replications. Each animal, except the controls, were treated 3 ml suspension of A. vera extract for 14 days by gavage. IGF-1 levels were measured using indirect ELISA. The result showed that the extract of A. vera significantly influenced the IGF-1 levels in DM group. The dose of 60 and 120 mg/day significantly raised the level of IGF-1 compared to C+. In NDM group the dose of 60 mg/ day significantly raised the level of IGF-1 compared to C-.


Keywords: Aloe vera, Rattus norvegicus, IGF-1, visceral fat, diabetes mellitus

References
Afaf, I. Abuelgasim, M.K.M. Osman, and B. Elmahdi. 2008. Effect of Aloe vera (Elsabar) Ethanolic Extract on Blood Glucose Level in Wistar Albino Rats. Journal of Applied Science Research. 4(12): 1841-1845.
Bedard, K., J. Strecko, K. Theriault, J. Bedard, C. Veyrat-Durebex, and P. Gaudreau. 2008. Effect of a High-Glocose Environment on the Pituiry Growth Hoemone-Realising Hormone Receptor: Type 1 Diabetes Compared with in vitro Glucoxity. Am J Physiol Endocrinol Metabolism. 294: E740 – E751.
Cock, I.E. 2011. Problems of Reproducibility and Efficacy of Bioassay Using Crude Extracts, with reference to Aloe vera. Pharmacognosy Communication. 1(1): 52-62.
Droge, W. 2002. Free Radicals in Physiological Control of Cell Function. Physiol Rev. 82: 47-97.
Erejuwa, O.O., S.A. Sulaiman, M.S.A. Wahab, S.K.N. Salam, M.S.M. Salleh, and S. Gurtu. 2011. Effect of Glibenclamide alone versus Glibenclamide and Honey on Oxidative Stress in Pancreas of Streptozotocin Induced Diabetic Rats. International Journal of Applied Research in Natural Products. 4(2): 1-10.
Fatchiyah. 2011. Glucomannan, Terapi Herbal Pengendalian Gula Darah pada Diabetes. http://fatchiyah.lecture.ub.ac.id/2011/06/glucomannan-terapi-herbal-pengendalian­gula-darah-pada-diabetes/, accessed 2 June 2012.
Forbes, J.M, M.T. Coughlan, and M.E. Cooper. 2008. Oxidative Stress as a Major Culprit in Kidney Disease in Dibetes. Diabetes. 52: 1446-1454.
Hamman, J.H. 2008. Composition and Applications of Aloe vera Leaf Gel. Molecules. 13: 1599-1616.
Hart, L.A., P.H. Nibbering, M.T. van den Barselaar, A.J. van den Berq, and R.P. Labadie. 1990. Effect of Low Molecular Constituents from Aloe vera Gel on Oxidative Metabolim and Cytotoxic and Bactericidal Activities of Human Neutrophils. International Journal Immunopharmacology. Abstract. 12(4): 427-34.
Joseph, B., and S.J. Raj. 2010. Pharmacognostic and Phytochemical Properties of Aloe Vera Linn An Overview. International Journal of Pharmaceutical Sciences Review and Research. 4(2): 106-110.
Khaing, T.A. 2011. Evaluation of the Antifungal and Antioxidant Activities of Leaf Extract of Aloe vera (Aloe barbadensis Miller). World Academy of Science, Engineering and Technology. 75: 610-612.
Kusmawati, A., and I.B. Pratiwi. 2009. Pengambilan Polisakarida Acemannan dari Aloe vera Menggunakan Etanol sebagai Pengendap. Semarang: Jurusan Teknik Kimia, Fakultas Teknik, Universitas Diponegoro.
Mali, R.A., S.R. Pattan, R.Y. Patil, A.M. Vayakole, P.S. Pedagonkar, U.D. Gharte, R.S. Jadhav, and D.G. Baheti. 2011. Need and Scope of Development of Aloe vera as Remedy Medicine For Skin Diseases. Pharmacologyonline. 1: 602-616.
Miladi, S., and M. Damak. 2008. In Vitro Antioxidant Activities of Aloe vera Leaf Skin Extracts. Journal del la Societe Chimique de Tunisie. 10: 101-109.
Padmadisastra, Y., Sidik, and S. Ajizah. 2003. Formulasi Cair Gel Lidah Buaya (Aloe vera Linn.) Sebagai Minuman Kesehatan. Simposium Nasional Kimia Bahan Alam III. 1-13.
Pi, J., Y. Bai, Q. Zhang, V. Wong, L.M. Floering, K. Daniel, J.M. Reece, J.T. Deeney, M.E. Anderson, B.E. Corkey, and S. Collins. 2007. Reactive Oxygen Species as a Signal Glucose-Stimulated Insulin Secretion. Diabetes. 56: 1783-1791.
Rajasekaran, S., K. Ravi, K. Sivagnanam, and S. Subraimanian. 2006. Benefical Effects of Aloe vera Leaf Gel Extract in Lipid Profile Status in Rats With Streptozotocin Diabetes. Clinical and Experimental Pharmacology an Physiology. 33: 232-237.
Riddel, M.C., and B.A. Perkins. 2006. Type 1 Diabetes and Vigorous Exercise: Applications of Exercise Physiologhy to Patient Management. Canadian Journal of Diabetes. 30(1). 63-71.
Saif-Ur-Rehman, S.A. Jafri, S. Hassan, I. Ahmed, and M. Naim. 2011. Study on Antidiabetic of Aloe vera Extract on Alloxan Induced Diabetic Rats. Libyan Agriculture Research Center Journal International. 2(1): 29-32.
Sujono, T.A., and A.S. Wahyuni. 2005. Pengaruh Decocta Daun Lidah Buaya (Aloe vera L) terhadap Kadar Glukosa Darah Kelinci yang Dibebani Glukosa. Journal Penelitian Sains & Teknologi. 6(1): 26-34.
World Health Organization (WHO). 2010. Global Status Report on Noncommunicable Diseases 2010. Geneva: WHO
World Health Organization (WHO). 2011a. Diabetes. http://www.who.int/mediacentre/ factsheets/fs312/en/index.html, accessed 31 May 2012.
World Health Organization (WHO). 2011b. Noncommunicable Disease in the South-East Asia Region. New Delhi: WHO.
Yeggnisetty, R.M., Ramachandraiahgari, S.R. Somesula, P.J. Adi, I.S. Mannur, and B.M. Enamala. 2012. Protective role of Ethanolic Extract of Aloe vera Antioxidant Properties on Liver and Kidney of Streptozotocin-Induced Diabetic Rats. Digest Journal of Nanomaterials and Biostructures. 7(1): 175-184.