Actividad Inhibitoria De La Hialuronidasa Del Extracto Hidroalcohólico De Piper Peltatum/Hyaluronidase Inhibitor Activity in Hydroalcoholic Extracts of Piper Peltatum
Abstract
Ecuador se ubica en una zona geográfica privilegiada, presenta zonas climáticas bien definidas con características ambientales y geográficas únicas. Estas particularidades hacen que cada región posea sus propios ecosistemas, existiendo así muchas especies vegetales y animales sin investigar. La población hace uso de estos recursos para tratar diversas dolencias como por ejemplo, Piper peltatum utilizada en caso de mordedura de serpiente. El objetivo del estudio fue determinar si los extractos de esta especie presentaban efectos inhibitorios sobre la hialuronidasa, para lo cual se identificó los metabolitos secundarios presentes mediante el tamizaje fitoquímico, se cuantificó la cantidad de fenoles y flavonoides totales a través de métodos espectrofotométricos y se determinó el porcentaje de inhibición tanto sobre la hialuronidasa bovina como la hialuronidasa presente en el veneno de Naja naja atra y Botrox atrox. Se identificó en P. peltatum alcaloides con potencial actividad antimitótica, cumarinas, terpenos, compuestos fenólicos y flavonoides. La cantidad de fenoles totales fue de 14,49±1,2 mg equivalente de ácido gálico/g extracto seco y 14,53±0,208 mg equivalente de Quercetina/g extracto seco de flavonoides. Respecto a la actividad inhibitoria se concluyó que existe mayor inhibición sobre la hialuronidasa bovina, seguida del veneno de Naja naja atra y no de manera significativa sobre B. atrox.
Ecuador is located in a privileged geographical area, it has strongly defined climatic zones with unique environmental and geographical characteristics. These particularities mean that each region has its own ecosystems, so many plant and animal species exist without investigating. The population makes use of these resources to treat various ailments such as Piper peltatum used in case of snakebite. The objective of the study was to determine if extracts of this species has inhibitory effects on hyaluronidase. The secondary metabolites present were identified by phytochemical screening, the amount of phenols and total flavonoids was quantified by spectrophotometric methods, as well as the determination of the inhibition of bovine, Naja naja atra venom and Botrox atrox hyaluronidase. P. peltatum contains alkaloids with potential antimitotic activity, coumarins, terpenes, phenolic compounds and flavonoids. The amount of total phenols was 14.49 ± 1.2 mg gallic acid equivalent / g dry extract and 14.53 ± 0.208 mg Quercetin equivalent /g dry flavonoid extract. Regarding the inhibitory activity, it was concluded that there is greater inhibition on bovine hyaluronidase, followed by Naja naja atra venom and not significantly on B. atrox.
Palabras claves: Piper peltatum, hialuronidasa, Naja naja atra, Extracto, Bothrops atrox.
Keywords: Piper peltatum, hialuronidase, Naja naja atra, extract, Bothrops atrox.
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