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Camacho P, Dutra-Medeiros M, Salgueiro L, Sadio S, C. Rosa P. Manual Segmentation of 12 Layers of the Retina and Choroid through SD-OCT in Intermediate AMD: Repeatability and Reproducibility. JOVR [Internet]. 2021Jul.29 [cited 2024Apr.26];16(3):384–392. Available from: https://knepublishing.com/index.php/JOVR/article/view/9435

https://doi.org/10.18502/jovr.v16i3.9435

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authors

  • Pedro Camacho

    Pedro Camacho

    H&TRC – Health & Technology Research Center, ESTeSL – Escola Superior de Tecnologia da Saúde, Instituto Politécnico de Lisboa, Lisbon, Portugal
    https://orcid.org/0000-0002-2986-5652
  • Marco Dutra-Medeiros

    Marco Dutra-Medeiros

    Central Lisbon Hospital Center, Lisbon, Portugal
  • Luís Salgueiro

    Luís Salgueiro

    Ophtalmology Institute Dr. Gama Pinto, Lisbon, Portugal
  • Sílvia Sadio

    Sílvia Sadio

    Ophtalmology Institute Dr. Gama Pinto, Lisbon, Portugal
  • Paulo C. Rosa

    Paulo C. Rosa

    Ophtalmology Institute Dr. Gama Pinto, Lisbon, Portugal

Published Date

  • Jul 29, 2021
This journal is publishing all the articles under Creative Commons Attribution License CC BY 4.0.

Manual Segmentation of 12 Layers of the Retina and Choroid through SD-OCT in Intermediate AMD: Repeatability and Reproducibility

Journal of Ophthalmic and Vision Research (JOVR) / July–September 2021, Volume 16, Issue 3 / Pages 384–392

Abstract

Purpose: To evaluate the repeatability and reproducibility of the segmentation of 12 layers of the retina and the choroid, performed manually by SD-OCT, along the horizontal meridian at three different temporal moments, and to evaluate its concordance with the same measurements performed by two other operators in intermediate AMD.


Methods: A cross-sectional study of 40 eyes from 40 subjects with intermediate AMD was conducted. The segmentation was performed manually, using SD-OCT. The 169 measurements per eye were repeated at three time points to study the intra-operator variability. The same process was repeated a single time by two different trained operators for the inter-operator variability.


Results: Forty participants (28 women and 12 men) were enrolled in this study, with an average age of 76.4 ± 8.2 (range, 55–92 years). Overall, the maximum values of the various structures were found in the 3 mm of the macula. Intra-operator variability: the highest ICC values turned out to be discovered in thicker locations. Inter-operator variability: except correlation values of 0.826 (0.727; 0.898) obtained in the OPL (T2.5) and 0.634 (0.469; 0.771) obtained in the IPL (N2), all other correlation values were >0.92, in most cases approaching higher values like 0.98.


Conclusion: The measurements of several layers of the retina and the choroid achieved at 13 locations presented a good repeatability and reproducibility. Manual quantification is still an alternative for the weaknesses of automatic segmentation. Locations of greatest concordance should be those used for the clinical control and monitoring.

Keywords:

Data Accuracy, Diagnostic Imaging, Macular Degeneration

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