Journal of Infertility and Reproductive Biology

Transitioning the Journal of Infertility and Reproductive Biology (JIRB) to New Horizons

2025-02-07T12:57:19+00:00

The Journal of Infertility and Reproductive Biology ( JIRB) is a prestigious quarterly, peer-reviewed, open-access journal dedicated to advancing research in the fields of infertility and reproductive sciences. This editorial announces the transition of JIRB’s publishing operations from Dorma Journals Publisher to West Kazakhstan Marat Ospanov Medical University, effective from Volume 12, Number 4 (2024). The shift represents a strategic enhancement of the journal’s visibility and impact, aligning with the university’s robust academic and research capabilities. This editorial outlines the significance of the transition, the journal’s scope, and its continued commitment to high-quality, open-access research.

Comparison of Estrogen and/or Progesterone on the Oxidative Status in Different Female Cancer Cells

2025-02-12T12:03:17+00:00

Background: Oxidative stress has a key role in the development of most types of cancers. Among all the conditions which affect the antioxidant defenses, the effect of using oral contraception pills (OCP) or sex hormone therapy in women during menopause is still an unsolved issue.

Objectives: In the present study, the effect of estrogen (EST) alone, progesterone (PRO) alone and EST+PRO together on oxidative status in HeLa, MCF-7 and OVCAR-3 cell lines was investigated.

Methods: HeLa, MCF- 7 and OVCAR-3 cell lines were treated with 1μM of EST, PRO and EST+PRO confirmed by MTT and used for 24 hour treatment. Then, catalase (CAT), glutathione (GSH), glutathione peroxidase (GPX), glutathione reductase (GR) activities and Malondialdehyde (MDA) levels were assayed in the cell lysate.

Results: The results of the present study indicated that EST, PRO, and their combination were strongly able to increase the antioxidant enzymes activity compared with controls and significantly decreased the MDA levels in all three cell lines.

Conclusion: The data suggest that female sex hormones and OCPs might exert antioxidant effects in different cancerous organs.

Nutritional Intake and Lifestyle in Infertile Women with and without Polycystic Ovary Syndrome: A Case-control Study

2025-02-07T12:59:45+00:00

Background: Polycystic ovary syndrome (PCOS), the most common endocrine pathology in females of reproductive age worldwide, is a multifactorial disorder. Although obesity, lifestyle, depression, and nutrition are considered possible contributing factors to PCOS pathogenesis, the association between nutrient intake, clinical indices, and adipokines in PCOS women is not comprehensively elucidated. Therefore, the current study aimed to reveal the contribution of nutritional intake and lifestyle to the pathogenesis of the disease.

Methods: 90 infertile women, 45 with PCOS as cases and 45 without PCOS as controls, aged 25–40 years were enrolled in the study. Different questionnaires including the antioxidant food frequency (using Nut4 software), international physical activity, fast food intake, depression, and internet addiction questionnaires were completed by participants. Moreover, demographic characteristics, weight, height, BMI, and the serum levels of hormones, fast blood glucose, malondialdehyde (MDA), chemerin, vaspin, and omentin-1 were measured.

Results: No significant differences between the two groups were obtained regarding demographic characteristics, physical activity, depression, and fast food intake (p-value>0.05). Moreover, the intake of calories and macronutrients did not significantly differ between the two groups (p-value>0.05). However, androgens, AMH, LH, LH: FSH ratio, FBS, and MDA were significantly higher and estradiol was significantly lower in PCOS subjects compared to controls (p-value<0.001). Moreover, a significant correlation between nutritional parameters and PCOS indicators was observed (p-value<0.05).

Conclusion: The findings may suggest that nutrient intake crucially contributes to the pathogenesis of PCOS in infertile women through hyperandrogenism and weight gain.

Nanomedicine Approaches in Male Infertility Treatment: Targeted Drug Delivery and Sperm Function Enhancement

2025-02-07T13:00:25+00:00

Male infertility affects a significant portion of the global population, contributing to nearly half of all infertility cases. Common underlying causes include disruptions in spermatogenesis, hormonal imbalances, and genetic anomalies. Traditional treatments, such as hormone therapy, surgical interventions, and assisted reproductive technologies, often exhibit limited efficacy due to their invasive nature, systemic side effects, and high financial costs. This study explores nanomedicine’s potential as a targeted approach to address these limitations, particularly through advanced nanoparticle-based drug delivery systems aimed at enhancing sperm function and improving fertility outcomes. Various types of nanoparticles, including liposomes, polymer-based nanoparticles, and metal nanoparticles, are assessed for their abilities to deliver therapeutic agents directly to spermatogenic cells and support cells in the testes. By modulating critical cellular pathways necessary for sperm production and survival, nanoparticles offer enhanced therapeutic effects with minimized systemic exposure. Additionally, the study highlights the potential of nanoparticle applications in personalized reproductive medicine, allowing for tailored treatment approaches based on individual profiles. Although further clinical trials are needed to confirm efficacy and safety, nanoparticle-based therapies offer a promising path forward in the minimally invasive, targeted treatment of male infertility.

Fabrication a Natural 3D-scaffold by Mixing Collagen and Decellularized Mouse Liver Extracellular Matrix for Tissue Engineering

2025-02-07T13:05:39+00:00

Objective: Liver transplantation is the traditional method for patients who suffer from liver failure. Due to the lack of donor organs, bioengineered liver produced from whole liver decellularized scaffold can be a potential applicable method. The aim of this study was to fabricate and characterize a natural 3D-scaffold by mixing collagen scaffold and decellularized mouse liver extracellular matrix (ECM) for tissue engineering.

Methods and Materials: After washing and removing the blood from the livers completely, they were shaken at room tempreture at 200 rpm on an orbital shaker in deionized water (DW) for 30 min and then shaked in 1% SLES at 200 rpm for about 16-18 h. Thereafter, they were washed in 1% triton and followed by DW for several times. The livers were lyophilized and mixed with collagen. All the scaffolds were evaluated by scanning electron microscope and H&E staining. Scaffold porosity was also determined and cell viability was checked by MTT assay.

Results: The data showed that since SLES led to losing nuclear material, it prevented the degradation of the liver’s ECM ultrastructure. DNA and cell debris clearance were verified. Although cells survived on the decellularized liver scaffold, their growth rate was slower than when mixed with collagen.

Conclusion: Combining collagen with decellularized liver ECM provides a biologically relevant microenvironment that closely mimics native tissue chemistry and protect cell survival.